武汉地区耐利福平结核分枝杆菌rpoB基因突变分析  

Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis clinical isolates in Wuhan district,China

作　　者：马峻[1] 温子禄[2,3] 张朝宝 郭莉[4] 张舒林[3] 王洪海[5] 余晓丽[2] 

机构地区：[1]武汉市医疗救治中心检验科,武汉430032 [2]武汉工业学院生物与制药工程学院,武汉430023 [3]上海交通大学医学院病原检测研究室,上海200025 [4]武汉工业学院校医院,武汉430023 [5]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《临床检验杂志》2011年第8期626-628,共3页Chinese Journal of Clinical Laboratory Science

基　　金："十一五"国家科技重大专项<特大城市结核病综合防治模式研究>(2009ZX10003-017);"十一五"国家科技重大专项(2009ZX10004-313);武汉工业学院研究生创新基金项目(2010cx016)

摘　　要：目的探讨武汉地区结核分枝杆菌(MTB)利福平耐药株rpoB基因的突变特征。方法对76例MTB临床分离株包括rpoB核心区域81 bp碱基在内的428 bp碱基进行PCR测定,并进行DNA序列分析。结果 76例临床分离MTB中利福平耐药株56例,敏感株20例。耐药株中92.9%(52/56)存在突变,共涉及10个密码子的18种突变类型。531、526为常见突变位点,其突变率分别为57.7%(30/52)、19.2%(10/52);联合突变率为13.5%(7/52);同时发现了509位(AGC→AGA)新的突变类型和国内少见的517位CAG缺失类型。结论 rpoB基因突变在武汉地区利福平耐药MTB中广泛存在,并存在新的突变位点。Objective To characterize rpoB mutations in rifampin-resistant Mycobacterium tuberculosis clinical isolates in Wuhan district,China.Methods The rpoB gene fragment including 81 bp of RRDR（rifampin resistance determination region） was analyzed following sequencing.Of the 76 Mycobacterium tuberculosis strains,56 strains were resistant to rifampin,and 20 strains were rifampin-susceptible.Results Among the rifampin-resistant isolates 92.9%（52/56） carried mutations in the amplified region of rpoB gene,involving 18 different types of mutations in 10 codons.The mutations at codon 531 and 526 were more frequently observed with the rate of 57.7%（30/52） and 19.2%（10/52）,respectively.The combined mutations rate was identified as 13.5%（7/52）.A new mutation type 509（AGC→AGA） was found.The CAG deletion type of 517 which was rarely observed in China was identified.Conclusion The mutations in rpoB gene are widely existed in rifampin-resistant Mycobacterium tuberculosis clinical isolates in Wuhan district.New mutation type may be found in succession.

关 键 词：结核分枝杆菌 RPOB基因 耐药性 DNA序列分析 

分 类 号：R378[医药卫生—病原生物学]