荧光共振能量转移淬灭法测定维生素B_2的含量  

Fluorescence resonance energy transfer quenching for determination of vitamin B_2

作　　者：钟文英[1,2] 黄斌[1,2] 陈琳[1,2] 舒畅[1,2] 

机构地区：[1]中国药科大学分析化学教研室 [2]中国药科大学药物质量与安全预警教育部重点实验室,南京210009

出　　处：《中国药科大学学报》2011年第6期527-533,共7页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.20975050);江苏省"六大人才高峰"资助项目(No.FJ10120)~~

摘　　要：以L-谷胱甘肽(GSH)为稳定剂合成了性能良好的水溶性ZnSe量子点,并利用其与维生素B2(VB2)组成荧光共振能量转移体系,考察ZnSe量子点(供体)和VB2(受体)之间荧光共振能量转移的最佳条件,建立了荧光共振能量转移淬灭法测定VB2的新方法。VB2在1.54×10-8～2.77×10-7 mol/L浓度范围内与ZnSe量子点的荧光强度变化F0/F呈现良好线性关系(r=0.996 7),检测下限(S/N=3)为1.0×10-8 mol/L。将1.54×10-7 mol/L VB2标准溶液平行测定9次,RSD为1.2%。将该方法用于3个不同厂家生产的VB2的含量测定,用加标的方法测得平均回收率为99.28%～101.9%(n=6),结果满意。相比紫外分光光度法,本方法测得VB2的含量更接近于标示量,这为痕量VB2的含量测定提供了一种简便灵敏的方法。Water-soluble ZnSe quantum dots（QDs） were synthesized with L-glutathione（GSH） as stabilizer.These QDs were applied for ultrasensitive vitamin B2（VB2） detection.It mainly discussed the FRET system constituted by the water-soluble ZnSe（donor） and VB2（receptor）.The optimum conditions for FRET and established a VB2 determination method based on the FRET principle were studied.The extent of fluorescence quenching（F0/F） of ZnSe QDs was linearly proportional to the trace concentration of VB2 from 1.54×10-8 to 2.77×10-7mol/L with a correlation coefficient of 0.996 7,a detection limit（S/N=3） of 1.0×10-8mol/L was obtained with the relative standard deviation of 1.2%（1.54×10-7 mol/L,n=9）.A novel method for selective detection of VB2 by utilizing the prepared QDs as fluorescence probes has been developed,based on the quenching fluorescence of ZnSe QDs.The proposed method was successfully applied to the determination of VB2 tablets from three different factors.The results were satisfactory and the recoveries could fall in 99.28%-101.9%（n=6）.The results were reliable,good agreement compare to those of UV-Vis absorption spectrometry.It was concluded that a novel and simple method based on fluorescence resonance energy transfer quenching theory was proposed for the determination of trace vitamin B2.

关 键 词：ZNSE量子点 荧光共振能量转移 维生素B2 荧光淬灭 

分 类 号：R927.2[医药卫生—药学]