紫杉醇高产菌发酵产物的分离、纯化和鉴定  

Isolation,purification and identification of paclitaxel from the fermented extracts of fungus ST026(Altemaria alternate var. monosporus)

作　　者：程首先 王荣华 陈杰鹏 段丽丽 邱雪莲 

机构地区：[1]汕头双骏生物科技有限公司,汕头515071

出　　处：《中国药科大学学报》2011年第6期570-572,共3页Journal of China Pharmaceutical University

基　　金：国家科技部资助项目(No.99C26214400050);广东省科技厅资助项目(No.99BO-1801G);广东省科技厅粤港招投标项目(No.200649841003)~~

摘　　要：真菌ST026(Altemaria alternate var.monosporus)的发酵产物,经过常压正相硅胶柱,中压正相硅胶柱,中压反相ODS柱色谱和重结晶等4步纯化得紫杉醇(纯度99.0%),4步总收率0.003 9%(以发酵浸膏计),紫杉醇的回收率为38.5%。Four steps,including normal silica gel chromatography,medium pressure silica gel chromatography,medium pressure ODS RP-chromatography and recrystalization,were used to isolate and purify paclitaxel from the fermented extracts of ST026（Altemaria alternate var.monosporus）.The total yield of paclitaxel was 0.003 9% with the purity of 99.0% and the recovery of of 38.5%.

关 键 词：真菌 发酵 纯化 鉴定 紫杉醇 维泰醇 

分 类 号：R284.1[医药卫生—中药学]