赣江中游四大家鱼遗传多样性的ISSR分析  

Study of Four Major Chinese Carps with Inter Simple Sequence Repeat(ISSR) Markers in the Middle Reach of Ganjiang River

作　　者：张建铭 吴志强[2] 胡茂林[3] 曾庆祥 

机构地区：[1]江西省赣州市水产研究所,江西赣州341000 [2]桂林理工大学环境科学与工程学院,广西桂林541001 [3]南昌大学生命科学与食品工程学院,江西南昌330031

出　　处：《水生态学杂志》2011年第5期69-73,共5页Journal of Hydroecology

基　　金：国家自然科学基金(30760188)资助

摘　　要：利用ISSR分子标记技术对赣江中游四大家鱼的遗传多样性进行分析,青鱼(Mylopharyngodon piceus)、草鱼(Ctenopharyngodon idellus)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)分别扩增出47、74、55、59个位点,其中多态性位点数分别为33、50、29、30,多态性位点比例分别为70.21%、67.57%、52.73%、51.72%。青鱼的Nei's基因多样性和Shannon信息指数分别为0.2606和0.3846,草鱼为0.2391和0.3560,鲢为0.1858和0.2769,鳙为0.1908和0.2827。结果显示,赣江四大家鱼群体均存在一定的遗传变异空间,其遗传多样性相对较丰富。The colonial genetic variations in four major chinese carps were analyzed with ISSR, which generated 47 （ black carp） , 74 （ grass carp） , 55 （ silver carp） and 59 （ bighead carp） bands. The number of polymorphie band were 33,50, 29, 30, and the pecerentage of polmoryphism were 70.21%, 67.57%, 52.73%, 51.72%. The index of Nei＇s gene diversity and Genetic diversity quantified by Shannon Index varied of black carp were 0. 2606 and 0. 3846, grass carp were 0. 2391 and 0. 3560, silver carp were 0. 1858 and 0. 2769, bighead carp were 0. 1908 and 0. 2827. The experiment results showed the heredity and variation space was a little larger in the four major Chinese carps in Xiajiaug reach of Ganjiang River, their colonial genetic diversity were abundant.

关 键 词：赣江中游 四大家鱼 ISSR 

分 类 号：Q343[生物学—遗传学]