蛇苔过氧化物酶基因的序列分析与功能预测  

Sequence analysis and function predication of poroxidase from Conocephalum conicum

作　　者：李茹兰[1,2] 边春象[2] 阮期平[2] 彭正松[1] 姜立春[2] 刘美[2] 

机构地区：[1]西华师范大学生命科学学院,南充637000 [2]绵阳师范学院分子生物学与生物制药重点实验室,绵阳621000

出　　处：《广西植物》2011年第6期806-812,共7页Guihaia

基　　金：绵阳师范学院校级科研基金(2011A05)~~

摘　　要：过氧化物酶在生物界分布极广,在细胞代谢的氧化还原过程中起重要的作用。为深入研究蛇苔过氧化物酶的理化性质及功能,对其同源性,亚细胞定位,结构和功能等进行了生物信息学预测和分析。结果表明:该序列有1050bp长的开放阅读框,编码349个氨基酸,与拟南芥等的POD相似性较高(相似性为71%)。预测R68、F71、H72、P170、H200和D276为该酶的活性位点,C41与C122、C74与C79、C128与C328、C207与C239形成四对二硫键,对蛋白质的功能、天然构象和稳定性有重要作用,为研究苔类植物过氧化物酶的作用机制和功能等奠定了基础。Peroxidases is widespread in biological world. It plays an important role in the cell redox metabolism. In or- der to luther study the characteristics and functions of Conocephalum conicum poroxidase, we performed bioinformat- ies analysis of its homology, subellular location, tertiary structure, function and so on. The results showed that the sequence had a complete open read frame of about 1 050 bp and encodes 349 amino acids,and the sequence had similar POD with that of Arabidopsis thaliana （the similarity beween them is 71%）. This sequence was predicated to be C. conicum poroxidase which belonged to the poroxidase super family, and R68, F71, H72, P170, H200 and D276 to be the C. conicum POD＇s active sites in which the four groups of disulfide bonds：CA and C122,C74 and C79,C128 and C328,C207 and C239 played a very important role in maintaining the protein＇s function, natural conformation and stability. This study would set an important foundation for the study of the interaction mechanisms and functions of the C. conicum poroxidase.

关 键 词：过氧化物酶 蛇苔 序列分析 功能预测 

分 类 号：Q943[生物学—植物学]