烟草叶片蛋白3种提取方法的比较研究  

Comparison Study on Three Methods for Tobacco Leaf Protein Extraction

作　　者：韦玉梅[1,2] 李佳素[1] 周杰[1] 王春台[1] 刘学群[1] 程钢[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《湖北农业科学》2011年第23期4968-4970,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30871318);中南民族大学自然科学基金项目(YZZ08006);中南民族大学植物发育遗传学术团队课题资助

摘　　要：使用酚/SDS法、Tris-HCl法及TCA/丙酮沉淀法提取烟草(Nicotiana tabacum)叶片蛋白,从终产物形式、颜色、提取时间、蛋白产量、SDS-PAGE电泳图谱等方面对3种方法进行比较,对提取的烟草叶片蛋白进行Western Blotting检测来评价3种方法提取蛋白的效果。结果表明,与2种常用方法相比,酚/SDS法具有快速、操作方便等特点,提取的蛋白纯度高、种类丰富,可用于Western Blotting检测,是一种合适的提取植物组织中蛋白的方法。Protein was extracted from tobacco（Nicotiana tabacum） leaves by three methods,phenol/SDS method,Tris-HCl method and TCA/acetone precipitation method.The extracting efficiency of the three methods was evaluated through the color and form of product,extraction time,protein yield and SDS-PAGE.Furthermore,the extracted protein was detected by Western Blotting.The results showed that compared with the other two methods,phenol/SDS method was faster and more convenient,and the protein extracted was with high quality,rich in protein species,and suitable for Western Blotting,thus was an appropriate method for extracting protein from plant tissue.

关 键 词：烟草(Nicotiana tabacum)叶片 蛋白 Tris-HCl法 TCA/丙酮沉淀法 酚/SDS法 

分 类 号：S572[农业科学—烟草工业] Q51[农业科学—作物学]