利用Affymetrix芯片分析民猪冷应激后肌肉组织的基因表达谱  

Gene Expression Analysis upon Cold Induced by Affymetrix Pig Chip

作　　者：张冬杰[1] 刘娣 别墅[1] 孙洪涛[1] 汪亮[1] 王文涛[1] 何鑫淼[1] 

机构地区：[1]黑龙江省农业科学院,哈尔滨150086

出　　处：《畜牧兽医学报》2011年第12期1661-1665,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31072020)

摘　　要：冷应激可引起动物生产性能下降,诱发多种疾病,甚至造成死亡。民猪是我国北部地区特有的一个抗寒品种,为了研究冷应激下基因的表达调控机制,本研究利用Affymetrix猪基因芯片对民猪冷应激后肌肉组织内表达发生变化的基因进行了高通量的检测。结果,共检测到差异基因34个,其中下调表达的基因27个,上调表达的基因7个。在下调表达的基因中有7个(OAS1、IRG6、ISG20、DDX58、IFITl、ISG15、CXCL9)与干扰素诱导相关,1个(FST)与生长性状相关,2个(MX1、PG-2)与抗病毒有关,2个(S100A9、Numb)与细胞生长和凋亡有关;上调表达的基因功能还不清楚,推测它们可能与提高机体的抗寒性有关。对其中5个基因进行了Real-time PCR分析,证实它们都受到了温度的调节,与芯片检测结果吻合。结果提示,重度冷应激会造成机体免疫力下降,生长减缓。Cold stress can cause animal performance descending,induce various diseases,even result in death.Min pig is a unique anti-cold breed in north region of China.In order to study regulation mechanisms of gene expression under cold stress,gene profiling was performed using Affymetrix pig chip on the basis of equally mixed RNA samples from 2 treatments.Thirty-four differentially expressed genes were detected,among them,twenty-seven genes were down-regulated and seven genes were up-regulated.Among down-regulated genes,seven genes（OAS1,IRG6,ISG20,DDX58,IFIT1,ISG15,CXCL9） were associated with interferon induction,one（FST） was associated with growth,two（MX1,PG-2） were associated with anti-virus,two（S100A9,Numb） were associated with cell growth and apoptosis.But the specific functions of the up-regulated genes were unknown,which may have relationship to animal cold resistant.Several genes were selected for Real-time PCR analysis,and the results showed that their expression patterns were consistent with the result of gene chip hybridization.The severe cold stress could cause immunity declining and growth slowing.

关 键 词：民猪 冷应激 基因芯片 REAL-TIMEPCR 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]