2009—2010年山东地区蛋鸡禽白血病病原学和血清学调查  

Etiology and Serology Investigation of Avian Leukosis form the Different Regions of Shandong Province of China in 2009-2010

作　　者：倪伟[1] 孙洪磊[1] 曲悦[1] 杨凤[1] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2011年第12期1738-1742,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家农业性行业(农业)科研专项(200803019)

摘　　要：为了解山东地区蛋鸡禽白血病(Avian Leukosis,AL)的流行病学情况,从泰安、临沂、青岛、聊城等4个地区的祖代种鸡场及其具有亲缘关系的父母代、商品代鸡场采集1 827份血清,其中祖代鸡血清597份、父母代鸡血清710份、商品代鸡血清520份;并采集1 209份泄殖腔棉拭子,其中祖代鸡棉拭子597份、父母代鸡棉拭子460份、商品代鸡棉拭子152份。通过ELISA检测试剂盒进行了血清的ALV-J、ALV-AB抗体检测、泄殖腔棉拭子的P27抗原检测。结果显示:祖代鸡ALV-J抗体、ALV-AB抗体、P27抗原的平均阳性率分别为0、0.84%、17.93%,其中不同地区P27抗原阳性率存在较大差异,泰安、聊城、青岛地区P27抗原阳性率分别为52.5%、0.58%、0;父母代鸡ALV-J抗体、ALV-AB抗体、P27抗原的平均阳性率分别为8.45%、3.10%、30.48%,不同地区之间三者差别较大;而商品代鸡ALV-J抗体、ALV-AB抗体、P27抗原的平均阳性率分别为13.46%、8.65%、18.42%,不同地区之间三者也存在较大的差别。祖代、父母代及商品代间抗体或抗原阳性率高低的明显相关性,提示存在垂直感染的可能性。山东地区祖代种鸡未检测到ALV-J抗体,ALV-AB抗体检出率极低,仅为0.84%,但个别祖代鸡场P27抗原的阳性率极高,可能存在免疫耐受感染;父母代、商品代鸡群抗体阳性率和抗原阳性率均比祖代高,说明它们已有ALV-J、ALV-AB的感染;父母代及商品代鸡群ALV-J抗体水平都高于ALV-AB抗体水平,说明山东省内AL以ALV-J感染为主,应注意研究其传播途径,加强检疫净化。In order to clarify the prevalence about etiology and serology of the AL in Shandong Province,1 827 samples of serum samples were collected from four regions,Tai＇an,Liaocheng,Qingdao and Linyi,in Shandong Province,including 597 samples of grandparent layer serum,710 samples of parent layer serum and 520 samples of commercial layer serum;at the same time,1 209 samples of cotton swabs were collected from these regions above,including 597 samples of grandparent layer swabs,460 samples of parent layer swabs and 172 samples of commercial layer swabs.Serums were tested for ALV-J and ALV-AB antibody and cotton swabs were tested for P27 antigen through ELISA.Result were as follows： The antibody of ALV-J,ALV-AB and antigen of p27 of grandparent layer is 0,0.84%,17.93% respectively,the diversity is big in different regions of P27 and the rate of antigen of Tai＇an,Liaocheng,Qingdao is 52.5%,0.58%,0 respectively.The antibody of ALV-J,ALV-AB and antigen of P27 of parent layer is 8.45%,3.10%,30.48% respectively,and the diversity is big in different regions among the rate of antibody of ALV-J and ALV-AB and antigen of P27.The antibody of ALV-J,ALV-AB and antigen of p27 of commercial layer is 13.46%,8.65%,18.42% respectively,and the diversity is also big in different regions among the rate of antibody of ALV-J and ALV-AB and antigen of P27.The evidence relative of antibody positive rate and antigen positive rate in Grandparent,Parent and Commercial chickens reveals that the possibility about existence of vertical infection.The ALV-J antibody in Shandong Province of Granparent chickens weren＇t detected,and ALV-AB antibody is very low（0.84%）,but the positive rate of antigen of P27 is great in some grandparent layer,we conjectured that there probably existed immunological tolerance infection;the positive rate of antibody and antigen of Parent and Commercial chickens were all higher than that of Granparent chickens,which illuminated that there has been ALV-J and ALV-AB infection in them;at the same time the ALV-J antibod

关 键 词：蛋鸡 ELISA抗体检测 抗原检测 血清学调查 

分 类 号：S851.38[农业科学—预防兽医学] S858.315.3[农业科学—兽医学]