均匀设计法优选白花刺参的提取工艺研究  

Optimization of extracting condition for Morina nepalensis by uniform design

作　　者：吴春蕾[1] 刘圆[1] 张志锋[1] 徐彦[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041

出　　处：《中草药》2011年第12期2447-2450,共4页Chinese Traditional and Herbal Drugs

基　　金：四川省科技厅应用基础研究项目(2009JY0017);西南民族大学中央高校基本科研业务费专项资金资助(09NZYZJ01)

摘　　要：目的优选白花刺参的最佳回流提取工艺条件。方法采用UV法和HPLC法测定总皂苷及刺参苷J的量,以总皂苷提取率、刺参苷J提取率和干浸膏收率为综合评价指标,采用U8(84)均匀设计表设计试验,考察乙醇体积分数、乙醇用量、提取次数、提取时间4种因素对总皂苷提取的影响。结果白花刺参的最佳提取工艺参数:80%乙醇回流提取2次,每次1 h。结论均匀设计法优选出的白花刺参总皂苷的提取工艺合理可行,为白花刺参总皂苷的进一步研究提供参考。Objective To optimize the extractive condition of Morina nepalensis by hot refluxing.Methods HPLC and UV were established to determine the monepaloside J and total saponins.U8（84） Uniform design form was selected as the experiments’ schedule,the total saponins,monepaloside J and the yield was used as evaluation indicator to and the ethanol concentration,amount of extraction solvent added,extractive time and times of extraction was used as the 4 influencing factors.Results The optimum condition was the extraction in 80% ethanol,two times and 1 h.Conclusion The optimal extracting method of the total saponins of M.nepalensis is suitable and feasible by uniform design.The study could provide scientific references for further investigation of M.nepalensis.

关 键 词：白花刺参 均匀设计 总皂苷 刺参苷J 提取工艺 

分 类 号：R284.2[医药卫生—中药学]