莱菔硫烷诱导人肝癌HepG2细胞凋亡与Fas/FasL途径的相关性研究  

Correlation between sulforaphane-induced human liver cancer HepG2 cell apoptosis and Fas/FasL pathway

作　　者：季宇彬[1,2] 张志坚[1,2] 邹翔[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2011年第12期2489-2491,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30300284);黑龙江省自然科学基金重大项目(ZJY03-04D200802)

摘　　要：目的研究莱菔硫烷对人肝癌HepG2细胞Fas、FasL、Bid蛋白表达的影响,探讨其诱导HepG2细胞凋亡过程中Fas/FasL途径的作用。方法 Caspase-8试剂盒检测莱菔硫烷对HepG2细胞Caspase-8活性的影响;流式细胞仪检测莱菔硫烷对HepG2细胞中Fas、FasL、Bid蛋白表达的影响。结果莱菔硫烷10、20、40μmol/L作用于HepG2细胞48 h,可显著升高HepG2细胞Caspase-8活性(P<0.01);可使HepG2细胞Fas、FasL蛋白的表达显著升高(P<0.01);莱菔硫烷20、40μmol/L时,可显著提高Bid蛋白的表达(P<0.01),上述作用均呈剂量相关性。结论激活Fas/FasL途径可能是莱菔硫烷诱导人肝癌HepG2细胞凋亡的重要机制之一。Objective To study the effect of sulforaphane on Fas,FasL,and Bid protein expression in HepG2 cells,and to explore the effect of Fas/FasL pathway on sulforaphane-induced apoptosis in HepG2 cells.Methods The effect of sulforaphane on Caspase-8 activity in HepG2 cells was detected by Caspase-8 Activity Assay Kit.Flow cytometer was used to analysis of Fas,FasL,and Bid protein expression in HepG2 cells.Results After treated by sulforaphane for 48 h,the Caspase-8 activity was effectively increased（P0.01）.At the doses of 10,20 and 40 μmol/L,the effect of sulforaphane could significantly increase the Fas and FasL protein expression in HepG2 cells in a dose dependent manner,while at the doses of 20 and 40 μmol/L,sulforaphane could increase the Bid protein expression.Conclusion Activating the Fas/FasL pathway may be one of the role mechanism of sulforaphane inducing the apoptosis of HepG2 cells.

关 键 词：莱菔硫烷 人肝癌细胞HEPG2 细胞凋亡 FAS/FASL途径 抗肿瘤 

分 类 号：R282.710.5[医药卫生—中药学]