激发子诱导下合作杨细胞钙、氢离子流的振荡  

Calcium and Proton Flux Oscillations Induced by Elicitors in P.simonii×P.pyramidalis "Opera 8277" Cells

作　　者：高海波[1,2] 沈应柏[1] 

机构地区：[1]北京林业大学生命科学与技术学院,北京100083 [2]临沂大学生命科学学院,山东临沂276002

出　　处：《西北农业学报》2011年第9期155-159,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金面上项目(30871727)

摘　　要：利用非损伤微测技术研究合作杨细胞受到水杨酸甲酯和茉莉酸甲酯2种激发子诱导后,细胞H+、Ca2+流的变化。结果表明,2种激发子均诱导合作杨细胞H+、Ca2+流变化、并呈现震荡的特征,在细胞整体水平上2种激发子诱导H+净外流,Ca2+净内流;2种激发子诱导的H+、Ca2+流的振荡周期基本一致。P.simonii×P.pyramidalis ＂Opera 8277＂ cells were treated with elicitor of MeSA and MeJA,and net ion fluxes of H＋ and Ca2＋ was measured by the method of Non-invasive micro-test technique.The result showed that two kinds of elicitor induced oscillations of H＋ flux and Ca2＋ flux in the whole-cell level,the integrated ion flux suggested that two kinds of elicitor induced H＋ net effulx and Ca2＋ net influx,autocorrelation function of ion flux showed that the period of oscillations were similar to each other under the treatment of two kind of elicitors.

关 键 词：合作杨 非损伤微测技术 离子流 激发子 

分 类 号：S792.11[农业科学—林木遗传育种]