山东肿足蕨高效液相色谱指纹图谱初步研究  

HPLC Fingerprint Spectrum of Hypodematium sinense lwatsuki

作　　者：张华丽[1] 朱军[1] 高桂花[1] 

机构地区：[1]济宁医学院药学院,山东日照276826

出　　处：《济宁医学院学报》2011年第6期389-391,共3页Journal of Jining Medical University

基　　金：济宁医学院2008年青年科研基金项目

摘　　要：目的建立山东肿足蕨药材的高效液相色谱指纹图谱,为其质量控制提供参考。方法采用高效液相色谱(HPLC)法,色谱柱VP-ODS,流速1ml/min,检测波长215nm,柱温25℃,用乙腈和水作为流动相,梯度洗脱,测定了10批山东肿足蕨样品。结果得到了山东肿足蕨HPLC指纹图谱。结论用文中的最佳条件可较全面地反映山东肿足蕨中的成分,为山东肿足蕨药材的质量控制提供参考。Objective HPLC fingerprint spectrum of Hypodematium Sinense Iwatsuri was established in order to provide reference for its quality control.Methods RP-HPLC （DAD） was used in the determination of Hypode- matium Sinense Iwatsuri by gradient elution under the following condition： column of VP-ODS, flow rate of lmL/ rain,detection wavelength of 215 nm, temperature of 25℃ and acetonitrile and water as mobile phase, 10 batches of Hypodematium Sinense Iwatsuri were determined.Results A preferable method for HPLC fingerprint determina- tion of the Hypodematium Sinense Iwatsuri was established. Conclusion A general acquaintance of the Hypodematium Sinense Iwatsuri can be obtained by using the preferable HPLC fingerprint determination method, which is useful for quality valuation of the Hypodematium Sinense Iwatsuri.

关 键 词：山东肿足蕨 高效液相色谱法 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]