α粒子辐照诱导拟南芥菜早期远程表观遗传改变研究  

Induction of Early Long-range Epigenetic Changes by α-irradiation in Arabidopsis thaliana Plants

作　　者：徐淑艳[1] 李方华[1] 王婷[1] 卞坡[1] 吴跃进[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,中国科学院合肥物质科学研究院技术生物与农业工程研究所,安徽合肥230031

出　　处：《原子核物理评论》2011年第4期479-484,共6页Nuclear Physics Review

基　　金：国家自然科学基金资助项目(10705029);中国科学院知识创新工程重要方向项目(KJCX2-YW-N34)~~

摘　　要：由于低能离子较低的组织穿透能力,其诱变机理一直是研究者争论的问题。近年来,本研究组的一系列研究工作已经证明在植物中存在辐射远程(诱变)效应,从一个新的角度解释了低能离子的诱变机理,然而依然无法解释低能离子辐照中的许多独特生物现象,而这些现象均具有明显的表观遗传学特性。以表观遗传学最具标志性特征的胞嘧啶甲基化为研究对象,以α粒子-拟南芥菜根辐照实验体系作为研究平台,检测了远程组织(器官)甲基化相关基因AtDML3的表达及特定基因片段的甲基化水平。研究证实,在植物个体水平辐射可以诱导远程表观遗传的变化,为进一步探索低能离子的诱变机理提供了新的思路。Along the way,the mutagenic mechanism of low-energy ions irradiation is a debatable issue.Recently,the existence of radiation induced long-range（mutagenic） effects in vivo in plants has been performed in a series of studies of our group,which account for the mutagenesis of low-energy ions irradiation in a new perspective.However,numerous distinct biology phenomena remain to be addressed,which bear obvious characteristics to epigenetic.In the present study,using the expression of methylation-related AtDML3 gene and methylation level of specific gene segments as end points,the methylation of cytosine,the most important feature of epigenetic,was investigated.It was shown that,in A.thaliana,root-loca-lized α-irradiation could induce epigenetic changes in aerial parts which avoided the direct irradiation.The radiation induced long-range epigenetic changes were confirmed in this study,which supplied innovative ideas for the further investigation of the mutagenetic mechanism of low-energy ions irradiation.

关 键 词：辐射诱导的远程表观遗传效应 甲基化 AtDML3基因 

分 类 号：Q947.9[生物学—植物学]