油茶岑软3号芽器官离体培养再生植株的研究  

Study on Sprout Culture in vitro of Camellia oleifera Cenruan 3

作　　者：王以红[1] 吴幼媚[1] 蔡玲[1] 陈博雯[1] 黄金使[1] 

机构地区：[1]广西林业科学研究院,广西南宁530002

出　　处：《西部林业科学》2011年第4期1-4,共4页Journal of West China Forestry Science

基　　金：广西自治区主席基金(09203-05);国家林业局推广项目([2010]TG02)

摘　　要：2009年1～12月通过对广西林业科学研究院采穗圃中的7年生油茶母树"岑软3号"的顶芽和茎段芽进行离体培养再生植株的研究,结果表明,无菌活外植体得率最高的月份是1年中的4月和10月;在改良MS+6-BA 2.5 mg/L+NAA0.3 mg/L+CH 600 mg/L上,顶芽诱导率69.8%,茎段芽诱导率53.6%;适宜油茶继代培养的培养基是改良MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,培养40天芽增殖系数4.9;单芽在1/2改良MS+IAA 3.0 mg/L+ABT6 0.3 mg/L上培养,培养20天后有根点突起,生根率达96.8%。The rapid propagation of plantlets of Camellia oleifera Cenruan 3 in vitro was studied using the stem segment with buds as the explant. The results showed that in April and October, the highest yield of aseptic living explant could be achieved. The medium for bud induction was MS ＋6 - BA2. 5 mg/L ＋ NAA0. 3 mg/L ＋ CH600 mg/L, with the terminal bud induction ratio and axillary bud induction ratio of 69.8 % and 53.6 % respectively. The me- dium for subculture was MS ＋ 6 - BA2. 0 mg,/L ＋ NAA0. 1 mg/L, with the bud proliferation coefficient of 4. 9 after 40 days＇ culture. The rooting rate of single bud on 1/2MS ＋ IAA 3.0 mg/L ＋ ABT60. 3 mg/L was 96. 8 %.

关 键 词：油茶 芽器官 再生植株 

分 类 号：S794.4[农业科学—林木遗传育种]