拟南芥悬浮细胞系的构建研究  

Study on Suspension Cell Line Establishment of Arabidopsis thaliana

作　　者：孔琼[1,2] 张燕[1] 张华茗[1] 王兴梅[1] 王云月[1] 杨红玉[3] 

机构地区：[1]云南农业大学植物保护学院,生物多样性与病害控制教育部重点实验室,云南昆明650201 [2]红河学院生物系,云南蒙自661100 [3]昆明学院生命科学与技术系,云南昆明650214

出　　处：《西部林业科学》2011年第4期12-16,共5页Journal of West China Forestry Science

基　　金：全球环境基金(GEF项目)“保护和利用作物遗传多样性控制病虫害发展可持续农业”;国家自然科学基金(30860121)共同资助

摘　　要：以拟南芥生态型种子为实验材料,研究其悬浮细胞愈伤组织继代培养效果。结果表明:在MS培养基上加入不同激素对拟南芥愈伤组织的诱导和继代培养产生显著影响,用最佳激素配比的MS培养基构建了良好的悬浮细胞培养体系。其中拟南芥愈伤组织最佳诱导配方为MS+3.0 mg.L-1 2,4-D+0.3 mg.L-1 6-BA;愈伤组织继代配方为MS+1.5 mg.L-1 2,4-D+0.3 mg.L-1 6-BA+1000 mg.L-1 CH;悬浮培养配方为MS+1.5 mg.L-1 2,4-D+0.3 mg.L-1 6-BA+1000 mg.L-1CH。Using the seeds of ecotype Columbia of Arabidopsis thaliana as the materials, the callus subcuhure effects of suspension cells were studied. The results indicated that adding different hormones to MS produced significant effects on induction and subculture of callus, the optimum hormone with MS was used to construct suspension cell line. The optimum media for callus induction, subculture of callus and suspension culture were MS ＋ 3.0 mg/L 2, 4-D ＋ 0.3 mg/L6-BA, MS ＋1.5 mg/L2, 4-D ＋ 0.3 mg/L6-BA ＋ 1 000 mg/L CH and MS ＋ 1.5 rag/L2, 4-D ＋ 0.3 mg/L6-BA ＋ 1000mg/LCHrespectively.

关 键 词：拟南芥 愈伤组织 悬浮细胞 植物激素 

分 类 号：Q943[生物学—植物学]