类风宁汤对大鼠佐剂性关节炎TNF-α、1L-1的影响  

The effects of Leifengning Decoction on IL-1 and TNF-a levels of rats with adjuvant-induced arthritis

作　　者：徐爱良[1] 彭韡[2] 丁志高[1] 蒋孟良[1] 谢勇[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省肿瘤医院,湖南长沙410013

出　　处：《湖南中医药大学学报》2011年第12期10-12,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(07JJ5044)

摘　　要：目的观察类风宁汤对大鼠佐剂性关节炎病理形态学及肿瘤坏死因子-a(INF-a)、白细胞介素-1(IL-1)的影响。方法将50只大鼠按照随机分组表分为正常组、模型组、类风宁低、高剂量组和阳性对照组。除正常组外,其他各组采用完全弗氏佐剂造模后,观察类风宁汤剂对大鼠佐剂性关节炎大鼠足关节病理形态改变和INF-a、IL-1的影响。结果类风宁汤能显著抑制佐剂性关节炎模型大鼠足跖滑膜、骨和软骨组织的破坏;与模型组比较,类风宁治疗组大鼠样本中INF-a、IL-1表达的灰度值(Grey)明显升高,光密度值(Density)明显降低,差异具有统计学意义(P<0.05)。结论类风宁汤对类风湿性关节炎具有一定的治疗作用,能够降低大鼠佐剂性关节炎INF-a、IL-1表达水平,减轻炎症反应,改善关节功能,对类风湿关节炎的临床治疗提供了可靠的依据。Objective To observe the effects of Leifengning Decoction on pathomorphological changes and levels of IL-1 and TNF-a of rats with adjuvant-induced arthritis.Methods 40 rats were randomly divided into blank control group, model group,treatment group with low dose Leifengning Decoction,treatment group with high dose Leifengning Decoction and positive control group.Except the blank control group,other groups were set as complete Freund＇s adjuvant arthritis models to observe the effects of Leifengning Decoction on pathomorphological changes and levels of IL-1 and TNF-a. Results Leifengning Decoction was effective in reducing the damages of toe synovium, bones and cartilage tissue of rats with adjuvant-induced arthritis.Comparing with model group,in treatment groups gray values of INF-a and IL-1 expression increased markedly and optical density value decreased evidently.Differences showed statistical significances （P〈0.05）. Conclusion Leifengning Decoction could reduce the expression of IL-1 and TNF-a of rats with adjuvant-induced arthritis and showed the treatment effects in relieving inflammatory reactions and improving functions of joints.These data offered the basis of clinical treatment of rheumatoid arthritis.

关 键 词：佐剂性关节炎 类风宁汤 白细胞介素-1 肿瘤坏死因子-A 大鼠 乌梢蛇 

分 类 号：R285.5[医药卫生—中药学]