C型肉毒梭菌的鉴定及其神经毒素基因全序列测定  

Identification of Clostridium botulinum Type C and Whole Gene Sequencing of Its Neurotoxin

作　　者：高建军[1] 张超[1] 毛晓燕[1] 曹馨匀[1] 

机构地区：[1]兰州生物制品研究所免疫室,兰州730046

出　　处：《中国生物制品学杂志》2011年第12期1425-1429,共5页Chinese Journal of Biologicals

摘　　要：目的对3株C型肉毒梭菌进行鉴定,并选择其中1株进行基因全序列测定。方法对3株C型肉毒梭菌(C6514、C314/91和CAXA)进行生化鉴定、检出试验、确证试验、毒力测定、毒素定型试验,并对C6514株进行神经毒素基因全序列测序。结果 3株菌均能发酵葡萄糖,不能发酵乳糖,不能发酵牛奶和形成吲哚;3株菌均为产毒株;C6514株毒力较高,为2×104 LD50/ml;加C型或混合型肉毒诊断血清或加明胶磷酸盐缓冲液(GPB)煮沸均可中和或灭活C型肉毒梭菌产生的毒素;BoNT/C测得的序列拼接后与GenBank中序列号为D90210的基因核苷酸和氨基酸序列同源性均为99%。结论 3株肉毒梭菌均为典型的C型肉毒梭菌。Objective To identify three strains of Clostridium botulinum type C and sequence the whole gene of neurotoxin of one strain.Methods C6514,C314 / 91 and CAXA strains of C.botulinum type C were subjected to biochemical assay,identity test,confirmation test,virulence test and toxin typing test,of which C6514 strain was sequenced for whole gene.Results The three strains were positive in glucose fermentation,but negative in milk fermentation,indole formation and lactose fermentation tests,all of which were toxigenic.The virulence of strain C6514 was 2 × 104 LD50 / ml,which was higher than those of the other two.The toxin produced by C.botulinum type C could be neutralized by type C or mixed type diagnostic serum for C.botulinum,or inactivated by boiling with geltin-phosphate buffer（GPB）.Both the homologies of nucleotides and amino acids of BoNT / C sequence after splicing were 99% to that of gene in GenBank（D90210）.Conclusion All the three strains were typical C.botulinum type C.

关 键 词：肉毒梭菌C型 生物学鉴定 神经毒素类 序列分析 

分 类 号：R378.83[医药卫生—病原生物学] Q78[医药卫生—基础医学]