环介导等温扩增反应的原理及应用  

Principle and Application of Loop-mediated Isothermal Amplification

作　　者：黄思佳[1] 解庭波[1] 严家新[1] 

机构地区：[1]武汉生物制品研究所基因工程室,武汉430060

出　　处：《中国生物制品学杂志》2011年第12期1511-1513,共3页Chinese Journal of Biologicals

基　　金：国家高技术研究发展计划(863计划)项目(2007AA-02Z402)

摘　　要：环介导等温扩增反应(Loop-mediated isothermal amplification,LAMP)是日本学者Notomi于2000年在NucleicAcids Res杂志上公开的一种新的基因诊断技术,该法操作简便,扩增效率及灵敏度高,已广泛应用于食品、临床、农业等领域,有望成为主流的基因诊断方法。本文对LAMP的反应特征及原理、优缺点、临床应用作一综述。Loop-mediated isothermal amplification（LAMP） is a novel technique for genetic diagnosis,which was published on Nucleic Acids Res in 2000 by Notomi,a Japanese scientist.It is a simple,efficient and sensitive DNA amplification method which has been widely used in various fields including food,clinic and agriculture,and might be a mainstream method for genetic diagnosis.This paper reviews the reaction characters,principle,advantage,disadvantage as well as clinical application of LAMP.

关 键 词：环介导等温扩增反应 核酸扩增技术 

分 类 号：Q789[生物学—分子生物学]