不同年龄健康人群肠道微生态差异比较  

Comparison of changes of intestinal microecology between different age groups in the healthy people

作　　者：李素军[1] 徐哲荣[1] 鲁海峰[1] 杨云梅[1] 

机构地区：[1]浙江大学医学院附属第一医院干部病房,杭州310003

出　　处：《中华老年医学杂志》2011年第12期990-993,共4页Chinese Journal of Geriatrics

基　　金：国家重点基础研究发展规划（973）项目（2007CB513008）

摘　　要：目的分析不同年龄健康人群肠道微生态的差异，探讨年龄与肠道菌群组成变化的相关性。方法收集少年组、成年组和老年组健康人群的粪样，对其主要菌群进行细菌培养和计数，采用荧光定量PCR技术分析其主要菌群在属水平上的定性和定量变化，计算其反映肠道定植抗力的指标B／E值（双歧杆菌与肠杆菌数值比）。结果粪便细菌培养结果显示，成年组和老年组的双歧杆菌数量较少年组明显减少（均P〈0．01），乳酸菌数量也明显减少（分别为P〈0．01、P〈0．05）；老年组类杆菌和肠杆菌数量较少年组和成年组均显著增加（均P〈0．01）；成年组和老年组肠球菌数量较少年组明显增加（分别为P〈0．05、P〈0．01），老年组与成年组比较也明显增加（P〈0．05）；老年组的B／E值（0．88±0．13），较少年组（1．15±0．15）和成年组（1．01±0．20）显著减少（均P〈0．05）。用荧光定量PCR检测双歧杆菌、类杆菌、肠杆菌数量及B／E值结果差异同细菌培养结果相符；荧光定量PCR检测少年组与老年组的乳酸菌数量差异、少年组与成年组的肠球菌数量差异较培养结果更为显著（定量PCR结果P〈0．01；培养结果P〈0．05）。结论随着年龄的增长，肠道双歧杆菌和乳酸菌数量明显减少，类杆菌、肠杆菌、肠球菌数量显著增加，肠道定植抗力也不断下降。Objective To analyze the diversity of intestinal microecology in different healthy age groups and explore the relationship between age and the composition of intestinal microflora. Methods Fecal samples were collected from the children, adult and elderly groups. The main intestinal microflora were cultured and quantified, and the qualitative and quantitative changes in the level of category were determined by fluorescence quantitative PCR. The Bifidobacteria/ Enterobacteriaceae （B/E） ratio reflexing intestinal colonization resistance was also calculated. Results The germiculture of fecal samples indicated that the counts of Bifidobacteria and Lactobacillus （P〈0. 01 and P〈0.05, respectively） were significantly lower in the adult and aged groups than in children group. The number of Bacteriodes and Enterobacteriaceae in the elderly group were higher than in the children and the adult groups （all P〈0.01）. The counts of Enterococcus were increased in the adult and the aged groups （P〈0.05 and P〈0.01） compared with the children group, and were higher in the aged group than in the adult group （P〈 0.05 ）. The B/E ratio was significantly decreased in the aged group （0.88±0.13） as compared with the children （1.15±0.15） and the adult groups （ 1.01 ± 0.20 ） （ both P〈 0.01 ） , The diversities of Bifidobacteria, Bacteriodes, Enterobacteriaceae and B/E ratio by fluorescent quantification PCR were consistent with the results by germiculture technology. The diversity of Lactobacillus （P〈0.01） between the children and the aged groups as well as between the children group and the adult group significantly improved （P〈0.01 and P〈0.05 ） in PCR compared with the germiculture technology. Conclusions The numbers of intestinal Bifidobacterium and Lactobacillus are decreased with lower colonization resistance, while the numbers of Bacteriodes, Enterobacteriaceae and Enterococcus are increased with aging.

关 键 词：肠 细菌 集落计数 微生物 

分 类 号：R446.5[医药卫生—诊断学]