冬虫夏草对慢性阻塞性肺疾病大鼠树突状细胞功能的影响  

Influence of Cordyceps sinensis on cytokine profile of dendritic cells in chronic obstructive pulmonary rat model

作　　者：李洪涛[1] 张天托[1] 黄静[1] 刘慧[1] 川上和羲[2] 

机构地区：[1]中山大学附属第三医院呼吸内科,广东广州510630 [2]日本东北大学医学部

出　　处：《中国病理生理杂志》2011年第12期2372-2375,共4页Chinese Journal of Pathophysiology

基　　金：广东省中医药局基金资助项目(No.2009156);广东省医学科学基金资助项目(No.A2009194)

摘　　要：目的:研究冬虫夏草对慢性阻塞性肺疾病(COPD)大鼠树突状细胞(DCs)功能的影响及其分子机制。方法:将大鼠随机分为COPD模型组、冬虫夏草治疗组和正常对照组,观察各组大鼠气道病理改变,分离培养鼠DCs,ELISA法检测DCs培养上清液和DCs-T细胞共培养上清液中细胞因子含量。结果:与正常对照组相比,COPD模型组和冬虫夏草治疗组的平均肺泡计数减少(P<0.05),冬虫夏草治疗组高于COPD模型组,但差异无统计学意义(P>0.05)。冬虫夏草治疗组DCs-T细胞共培养后上清液中IFN-γ水平高于COPD模型组和正常对照组(P<0.05),IL-5水平各组无显著差异(P>0.05)。冬虫夏草治疗组DCs上清液中TNF-α和IL-12 p70水平高于COPD模型组和正常对照组(P<0.05)。结论:冬虫夏草可促使COPD大鼠DCs产生Th1型细胞因子,机制可能与DCs产生的IL-12 p70促进T细胞产生IFN-γ有关。AIM： To investigate the effect of Cordyceps sinensis（CS） on dendritic cells（DCs） in the rat model of chronic obstructive pulmonary disease（COPD）.METHODS： Eighteen Sprague-Dawley male rats were randomly divided into 3 groups： control group,COPD group and CS group.The rats in the latter 2 groups were exposed to cigarette smoking for 8 weeks with（CS group） or without（COPD group） CS treatment.The rats in control group were maintained under normal condition.After 8 weeks,the histological changes of the right lung were observed under microscope.The DCs from the 3 groups were harvested and the supernatants of DCs were analyzed for the levels of TNF-α and IL-12 p70 by commercially available ELISA kit.The DCs were then washed and cocultured in vitro with autologous T cells purified by a nylon cotton column.The supernatants of DCs-T coculture were collected after 72 h incubation,and analyzed for the levels of interleukin-5（IL-5） and interferon-γ（IFN-γ） by ELISA.RESULTS： Analysis of the rat lung parenchyma revealed a significant decrease in the mean alveolar number,an indicator of alveolar density,in COPD group（38±16） and CS group（48±9） in comparison with control group（62±8）.The mean alveolar number tended to be increased in CS group than that in COPD group,although this difference did not achieve statistical significance（P0.05）.The concentrations of TNF-α and IL-12 p70 in the culture supernatants of DCs and IFN-γ in the supernatants of DCs-T cocluture were up-regulated in CS group as compared with those in COPD group and control group（P0.05）.The level of IL-5 in the DCs-T coculture supernatants of the 3 groups did not show differences with statistical significance（P0.05）.CONCLUSION： The therapeutic effects of CS on COPD rats may be related to modulation of Th1 and Th2 cell functions.This effect is probably mediated through IL-12 p70 produced by DCs and Th1 cytokine IFN-γ produced by autologous T cells.

关 键 词：肺疾病 慢性阻塞性 冬虫夏草 树突状细胞 

分 类 号：R563.3[医药卫生—呼吸系统]