人生长激素对乳腺癌细胞增殖和凋亡的影响  

Effects of Human Growth Hormone on Proliferation and Apoptosis of Human Breast Cancer Cells

作　　者：许立生[1] 王水[2] 黄中晶[1] 沈恩超[1] 钱超[1] 

机构地区：[1]江苏大学附属医院乳腺外科,212001 [2]南京医科大学第一附属医院

出　　处：《天津医药》2011年第12期1141-1143,I0008,共4页Tianjin Medical Journal

摘　　要：目的:探讨人生长激素对人乳腺癌细胞株MCF-7增殖和凋亡的影响。方法:建立乳腺癌裸鼠移植瘤模型,将40只裸鼠随机分为:对照组、重组人生长激素(rhGH)组、5-氟尿嘧啶(5-FU)组和rhGH+5-FU组,各组腹腔注射给药,2周后观察各组药物对肿瘤生长的影响。分别采用MTT法和免疫组化检测肿瘤细胞增殖,TUNEI法检测移植瘤组织的细胞凋亡。结果:5-FU组和rhGH+5-FU组的肿瘤质量、PI及S期的人乳腺癌细胞数均明显小于对照组(P<0.01),AI、G0/G1期人乳腺癌细胞数均明显大于对照组(P<0.05或P<0.01);rhGH+5-FU组的PI及G2M期人乳腺癌细胞数均明显小于5-FU组(P<0.05)。结论:人生长激素在体内对人乳腺癌细胞既无明显的增殖作用,也无明显的凋亡作用,但与5-FU联用后能增强化疗对MCF-7乳腺癌细胞的效果,且具有协同作用。Objective： To study the effects of human growth hormone on proliferation and apoptosis in human breast cancer cells. Methods： The mouse xenograft model of breast cancer was established. Forty nude mice were randomly divided into four groups, control group, recombinant human growth hormone （rhGH） group, 5-fluorouracil （5-FU） group and rhGH＋5-FUgroup. Drugs were given intraperitoneally for 2 weeks in mice of 4 groups. The cell proliferation was detected by MTT and immunohistochemical methods. The apoptosis of human breast cells was detected by TUNEL assay. Results： The values of tumor mass, numbers of cells in PI and S phases were smaller, and numbers of cells in AI phase and G0/G1 phase were significantly increased, in 5-FU group and rhGH＋5-FU group compared with those of control group （P〈0.05 or P〈0.01）. Numbers of cells in PI phase and G2M phase were significantly decreased in rhGH＋5-FU group compared with those of 5-FU group （P〈0.05）. Conclusion： The exogenous human growth hormone has no obvious effect on proliferation and apoptosis in human breast cancer cells. But, r-hGH combined with 5-FU enhanced the chemotherapeutic sensitivity to human breast cancer cells.

关 键 词：人生长激素 乳腺肿瘤 细胞增殖 细胞凋亡 氟尿嘧啶 细胞周期 小鼠 近交BALBC 

分 类 号：R979.1[医药卫生—药品]