单因素方差分析优化桂枝茯苓胶囊中苦杏仁苷浸出率  

One–Way ANOVA Optimizing the Extract Technology of Amygdaloside in GZFL Capsules

作　　者：王红英 黄燕芬[2] 

机构地区：[1]兰溪市人民医院,浙江兰溪320000 [2]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中华中医药学刊》2011年第12期2700-2701,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅基金资助项目(20060721)

摘　　要：目的:优化乙醇对桂枝茯苓胶囊苦杏仁苷的浸出率。方法:以苦杏仁苷浸率为考核指标,单因素方差分析最佳浸出苦杏仁苷乙醇浓度。用不同浓度乙醇处理桂枝茯苓胶囊,高效液相色谱法测定各乙醇处理液中苦杏仁苷的浓度。色谱柱:(4.6mm ID×150mm,粒径5μm);流动相:甲醇和0.2%磷酸水(24∶76);检测波长:210nm;流速mL.min-1;柱温:室温;进样量15μL。结果:苦杏仁苷在0.3125～3.125μg范围内线性关系良好(r=0.9999),(n=6),60%乙醇对苦杏仁苷浸出率最高。结论:该方法重现性好,可作为桂枝茯苓胶囊乙醇浸出苦杏仁苷的简便方法。Objective：To optimize the extract technology of amygdaloside in GZFL capsules. Methods：Take amygdaloside content in extract as observation index, One Way ANOVA was used to study the factor of concentration of ethanol to extract amygdaloside in GZFL capsules. GZFL was treated with deferent concentration ethanol. Amygdaloside was determinated by HPLC. The column was Hypersil C18 （4.6 mm 150mm, 5 （m）. The mobile phase consisted of methanol and 0.2% phosphoric acid （24 ： 76） at a flow rate of 1mL. min-1. The detection wavelength was 210nm. Results： Amygdaloside had a good linearity in the range of 0. 3125 - 3. 125μg （ r =0. 9999 ）. The average recovery was 99.9%, RSD = 0.36%. 60% ethanol is the most suitable to extract Amygdaloside in GZFL capsules. Conclusion ： The method is convenient, accurate, and has good reproducibility. It can be used to extract Amygdaloside in GZFL capsules.

关 键 词：桂枝茯苓胶囊 HPLC法 单因素方差分析 苦杏仁苷 

分 类 号：R283[医药卫生—中药学]