广西产磨盘草药材中总黄酮的含量测定  

Total Flavonoids Determination in Abutilon indicum(L.) Sweet

作　　者：陈勇[1] 杨晨[1] 魏后超[1] 韦韬[1] 黄颖[1] 

机构地区：[1]广西中医学院药学院,广西南宁530001

出　　处：《中华中医药学刊》2011年第12期2721-2722,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：广西科技能力建设项目(桂科能07109004-01Z)

摘　　要：目的:建立磨盘草药材总黄酮的含量测定方法。方法:50%乙醇回流提取1h,以芦丁为对照品,采用紫外-可见分光光度法测定总黄酮含量。结果:本法在60min内测定稳定,芦丁的线性方程:Y=11.323X-0.0139,相关系数r=0.9997(n=6),平均加样回收率为98.47%(n=6),RSD=1.29%。结论:该方法简便、准确、稳定性好,可作为磨盘草药材的质量控制方法。Objective ： To establish the quality standard of total flavonoid in Abutilon indicum （ L. ） Sweet. Method ： We extracted the total flavonoid by 50 % of ethanol for one hour in water - bath, and used rutin as a reference substance, used UV spectrop hotometer for contentdetermination of flavonoids. Result ：The method was stable in 60 minute, the cali- bration curve of rutin at 510nm was Y = 11. 323X -0. 0139, r =0. 9997（ n =6）, and the average recovery was 98.47% （ n =6） with RSD of 1. 29%. Conclusion ： This method was simple accurate and sensitive. It can be used effectively for the quality control of this preparation.

关 键 词：磨盘草 总黄酮 紫外-可见分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学]