崂山奶山羊乙酰辅酶A羧化酶α基因(ACACA)多态性及其与泌乳性状的相关分析  

Association between Polymorphism of Promoter Ⅲ of Acetyl-CoA Carboxylases-α Gene(ACACA) and Milk Production Traits in Laoshan Dairy Goat

作　　者：王金凤[1] 王建民[1] 王桂芝[1] 陈珊珊[1] 王勇[1] 赵金山[2] 王建华[2] 程明[2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]青岛市畜牧兽医研究所,青岛266000

出　　处：《农业生物技术学报》2011年第6期1042-1050,共9页Journal of Agricultural Biotechnology

基　　金：国家公益性行业(农业)科研专项(No.201103038);国家转基因生物新品种培育科技重大专项(No.2009ZX08008-004B);山东省农业良种工程项目(No.20082010)共同资助

摘　　要：利用高效液相色谱法(denaturing high-performance liquid chromatography,DHPLC)和直接测序法,检测了174只崂山奶山羊(Capra hircus)泌乳母羊的ACACA基因启动子PⅢ位点多态性,采用最小二乘方法分析其与泌乳性状之间的关联效应。结果显示,在启动子PⅢ部分序列中共检测到3个SNPs,即第1206位的B/D,1255位的A/C和1322位的M/N,其等位基因和基因型频率在种羊场(F)和农户(P)两个群体中分布不均衡,均处于Hardy-Weinberg平衡状态(P>0.05)。在两个样本群中,大部分泌乳性状均存在显著的场别效应(P<0.01或P<0.05),而位点的基因型效应均不显著(P>0.05)。在F群体的1206位点上,等位基因B为乳蛋白率、乳糖率、非脂肪物性状的有利等位基因,BB基因型与BD基因型的乳蛋白率和乳糖率性状差异极显著(P<0.01),非脂肪物和乳密度性状差异显著(P<0.05),乳蛋白率、乳糖率和非脂肪物性状均存在显著的等位基因替代效应(P<0.05);而在P群体中,两种基因型之间的所有性状则差异不显著(P>0.05)。在F群体的1322位点上,等位基因N为乳糖率的有利等位基因,为300 d产奶量的不利等位基因,MN基因型与MM基因型的300 d产奶量和乳糖率性状差异显著(P<0.01或P<0.05),其等位基因替代效应分别为-52.22 kg和0.12%(P<0.01);而在P群体中,等位基因M为乳蛋白率、乳糖率、非脂肪物、乳密度的有利等位基因,基因型MM与MN在乳蛋白率、乳糖率、非脂肪物和乳密度性状均存在显著差异(P<0.05),乳糖率和非脂肪物的等位基因替代效应分别为-0.25%和-0.44%(P<0.05)。研究结果提示,ACACA基因启动子PⅢ相同等位基因在不同育种群体中,对乳蛋白率、乳糖率、非脂肪物、乳密度、300 d产奶量存在不同程度的影响。The current study was aimed to analyze the association of promoterⅢ in Acetyl-CoA carboxylases-α gene（ACACA） with milk traits in Laoshan dairy goat（Capra hircus）.Denaturing high-performance liquid chromatography（PCR-DHPLC） and direct sequencing were applied to identify SNPs in promoter Ⅲ of ACACA gene in Laoshan dairy goat population containing 174 samples,and least square method was performed to evaluate the association of polymorphism in promoter Ⅲ with milk traits.The SNPs have been identified as followed,B/D at 1206 bp,A/C at 1255 bp,M/N at 1322 bp.The distribution of alleles and genotypes in both Group F and Group P was not balanced,but still under Hardy-Weinberg equilibrium.The variation between two genotypes was not significant in the whole population,only the difference between different herbs existed.Further,the difference between different genotypes were analyzed in Group F and Group P,respectively.The 1206 site in F group,allele B had a positive effect on protein,lactose and non-fat rate,and the difference between genotypes BB and BD was statistically significant for protein rate and fat rate（P0.01）,for non-fat and milk density（P0.05）.The substitution effects for protein,lactose and non-fat rate were significant（P0.05）.While in P group,the difference between genotypes BD and BB for all milk traits was not significant（P0.05）.Allele N was the dominant allele for lactose rate,while this was the negative allele for milk yield at 1322 site in F group,and the difference between genotypes MN and MM was significant for milk yield（P 0.01） and for lactose rate（P0.05）.The substitution effects for lactose rate and milk yield were 0.12% and-52.22 kg（P0.01）,respectively.In P group,allele M was the dominant allele for protein rate,lactose rate,non-fat and milk density,the difference between genotypes MM and MN was significant for protein rate,lactose rate,non-fat and milk density（P0.05）.The substitution effects for lactose rate and non-fat were-0.25%,-0.44%（P0.05�

关 键 词：崂山奶山羊 乙酰辅酶A羧化酶α基因(ACACA) 高效液相色谱法 泌乳量 乳成分 

分 类 号：S823.2[农业科学—畜牧学]