牛性控精子体外受精及受精卵的体外培养  

In vitro Fertilization of Bovine Oocytes with Sexed Sperm and Zygote Culture

作　　者：赵晓娥[1] 马保华[1] 屈晓君[1] 刘闯[1] 赵晓鹏[1] 

机构地区：[1]西北农林科技大学动物医学院,农业部动物生物技术重点实验室,杨凌712100

出　　处：《农业生物技术学报》2011年第6期1070-1074,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2008AA101003)资助

摘　　要：为提高牛性别分离精子体外受精的效率,比较精卵共孵育8和22 h受精卵的体外发育能力,使用G-1/G-2序贯培养液和SOFaa培养液培养牛性控受精卵,筛选出一种适合牛性控胚胎体外培养的培养液。精卵共孵育22和8 h,牛性控受精卵的卵裂率分别为(55.9±4.52)%和(50.88±4.44)%,囊胚发育率分别为(35.05±5.02)%和(41.16±5.12)%,差异均不显著(P>0.05);精卵共孵育8 h组的囊胚孵化率(52.56±6.95)%显著高于22h组(39.81±6.38)%(P<0.05)。用SOFaa和G-1/G-2培养牛性控受精卵,其体外发育率无显著差异。但G-1/G-2液中受精卵发育到第7天时囊胚内细胞数(113.9±9.46)显著高于SOFaa组(102.0±9.97)(P<0.05)。性控精子与卵子共孵育8 h可提高牛性控受精卵的囊胚孵化率,G-1/G-2培养液更利于牛性控受精卵的体外培养。The purpose of this investigation was to improve the fertilization efficiency of bovine sexed sperm.In order to search an appropriate incubation time,we co-cultured bovine oocytes with sexed sperm for 8 and 22 h,respectively.Meanwhile,we compared effect of two mediums on developmental capacity of sexed zygotes,including G-1/G-2 sequential medium and SOFaa single medium.The results showed that the cleavage rates of sexed zygotes were（50.88±4.44）% and（55.9±4.52）%（P0.05） for 8 and 22 h incubation time,respectively.Furthermore,the blastocyst rates were（41.16±5.12）% and（35.05±5.02）%（P0.05）,respectively.However,the hatched blastocyst rate of sexed zygotes in 8 h incubation time was（52.56±6.95）%,obviously higher than that of 22 h（39.81±6.38）%（P0.05）.On other hand,in terms of the cleavage rate,the blastocyst rate and the hatched blastocyst rate,we found that there were no significantly difference between G-1/G-2 sequential medium and SOFaa single medium.Whereas,compared to the SOFaa medium,the sexed blastocysts cultured in G-1/G-2 medium had a larger cell number of blastocyst（113.9±9.46 vs 102.0±9.97）（P0.05）.These results suggest that reducing oocytes and sexed sperm incubation time can improve the hatched blastocyst rate of bovine sexed zygotes.According to cell number of blastocyst,G-1/G-2 sequential medium can be more suitable for in vitro culture of bovine sexed zygotes.

关 键 词：性别分离冻精 G-1/G-2培养液 精卵共孵育 体外受精 

分 类 号：S826[农业科学—畜牧学]