基于RAPD标记的福建省稻曲病菌遗传多样性分析  

Analysis of Genetic Diversity of Ustilaginoidea virens from Fujian Province Based on RAPD Markers

作　　者：杨秀娟[1] 王舒婷[2] 姚锦爱[1] 杜宜新[1] 陈福如[1] 

机构地区：[1]福建省农业科学院植物保护研究所,福建福州350013 [2]福建农林大学植物保护学院,福建福州350002

出　　处：《农业生物技术学报》2011年第6期1110-1119,共10页Journal of Agricultural Biotechnology

基　　金：福建省科技厅公益类科研专项(No.2010R1026-3);国家公益性行业(农业)科研专项(No.200903039-5);福建省科技创新平台建设项目(No.2009N2005)共同资助

摘　　要：为了解福建省稻曲病菌群体的遗传多样性和遗传组成,应用随机扩增多态性RAPD(randomamplified polymorphic DNA)技术分析了来自福建省不同水稻种植区的102个稻曲病菌(Ustilaginoidea virens)的遗传多样性。从100条随机引物中筛选出10条扩增带清晰、重复性好的引物进行稻曲病菌多态性扩增,共扩增出157条带,多态性条带比率为82.17%,遗传距离变化范围为0.02～0.67。遗传多样性分析表明,福建省稻曲病菌具有丰富的遗传多样性,相对于其它地区而言,福建闽西地区分离的菌株遗传多样性水平最高PPB=76.43,H=0.2212,I=0.3383),晚稻分离的菌株群体遗传多样性(PPB=91.08,H=0.2402,I=0.3655)高于早稻群体(PPB=63.06,H=0.1892,I=0.2870)。聚类分析显示,在遗传距离0.349水平上,供试的所有菌株可被划分成7个遗传聚类组(R1～R7),聚类组R1为优势聚类组,包含有80个菌株,其内又存有一些亚组。这些菌株的聚类与菌株的地理来源及水稻品种无明显相关性。但是在遗传距离0.330水平上,来自宁化的10个菌株可被明显划分成早稻群体和晚稻群体。初步分析认为菌株的地理来源、水稻品种及其生长季节是影响福建省稻曲病菌遗传多样性的主要因素,在稻曲病菌的遗传变异以及该病的发生和流行中可能起重要的作用。To know the genetic diversity and constitution of population of Ustilaginoidea virens in Fujian Province,the genetic diversity of 102 isolates on rice（Oryza sativa） collected from different rice growing areas in Fujian Province were assessed with RAPD（random amplified polymorphic DNA）.A total of 157 bands were amplified by 10 primers selected from 100 random primers,with the percentage of polymorphic bands of 82.17% and genetic distance between 0.02~0.67.The results showed that there were significant genetic diversity in the population of Fujian,the highest genetic diversity of population was observed at rice growing areas of Mingxi（PPB=76.43,H=0.2212,I=0.3383）in comparison to the population of the other areas,and the genetic diversity of population from late season（PPB=91.08,H=0.2402,I=0.3655） were more diverse than that of early season（PPB=63.06,H=0.1892,I=0.2870）.The result of cluster analysis revealed that 102 isolates tested could be classified into 7 genetic groups（R1 to R7） at 0.349 genetic distance level,and R1 was the predominant group contained 80 isolates in which existed some subgroups.And these groups had no obvious correlation with the geographic origin and the rice varieties origin of isolates.However,10 isolates from Ninghua could be classified into 2 genetic groups,early season and late season,at 0.330 genetic distance level.The preliminary analysis show that geographic origin of isolates,rice varieties and their growing season may be the main factors for the genetic diversity of U.virens in Fujian,and play an important role in genetic variation of the pathogen,and occurrence and prevalence of the rice false smut.

关 键 词：水稻 稻曲病 稻曲病菌 RAPD 遗传多样性 

分 类 号：S635.1[农业科学—蔬菜学]