IL-18启动子区-137G/C和-607C/A位点多态性与结核病易感性关系——Meta分析  

Association between the polymorphisms of interleukin-18 promoter gene-137 G/C and-607 C/A and the susceptibility of tuberculosis:a Meta-analysis

作　　者：张喆[1] 马红霞[1] 于学文[1] 王秋萍[1] 杨健[1] 郭佑民[1] 

机构地区：[1]西安交通大学医学院第一附属医院医学影像科,710061

出　　处：《中国防痨杂志》2011年第12期815-819,共5页Chinese Journal of Antituberculosis

摘　　要：目的应用Meta分析探讨IL-18基因启动子区单核苷酸多态性(single nucleotide polymorphism,SNP)等位基因位点-137和-607与结核病易感性的关系。方法收集采用序列特异性引物聚合酶链反应(PCR-SSP)及测序技术检测SNP位点-137和-607的研究,在严格的质量评价基础上,通过统计学方法应用Review Ma-nager 5.0软件进行了Meta分析。入选文献标准:(1)关于IL-18启动子区SNP-137G/C和(或-)607C/A与结核易感性关联的病例-对照研究;(2)对照组基因分布符合Hardy-Weinberg(H-W)遗传平衡定律;(3)原始资料完整;(4)文献中提供基因型频率或等位基因频率;(5)纳入文献语言为中文或英文;(6)多态性检测方法通过PCR-SSP及测序技术或SNaPshot SNP分型方法。文献排除标准:(1)重复报告的研究;(2)数据不清或等位基因不清的文献;(3)基于家系的研究;(4)对照组基因分布没有进行H-W遗传平衡检验的研究。结果 IL-18启动子区SNP-607C/A位点研究6项,患者695例,正常对照879例;SNP-137G/C位点研究5项,患者594例,正常对照781例。结核病组IL-18启动子区等位基因-137G和-607C与对照组相比,等位基因-137C和-607A的合并OR值分别为1.41(95%CI为1.15～1.74,P=0.001)和0.93(95%CI为0.81～1.08,P=0.36)。结论 IL-18启动子区SNP位点-137G/C与结核病易感性相关联,提示IL-18启动子SNP-(137)是结核病的易感位点之一;SNP位点-607C/A与结核病易感性之间关联无统计学意义。Objective To provide a quantitative summary in estimating the association between the polymorphisms of two loci in interleukin-18（IL-18） gene promoter and susceptibility of tuberculosis by Meta-analysis.Methods The polymorphisms of IL-18 promoter gene-137 G/C and-607 C/A were analyzed using the sequence specificity primer polymerase chain reaction（PCR-SSP） and gene sequencing analysis.Based on the strict quality evaluation,statistics was carried out for Meta analysis by Review Manager 5.0 software.Inclusion criteria were as follow：（1） the studies on the association between the polymorphisms of IL-18 promoter gene-137 G/C and-607 C/A and the tuberculosis susceptibility;（2） the gene distribution of the control group coincides to the law of genetic equilibrium of Hardy-Weinberg;（3） the original material was complete;（4） the study provided the genotype frequency or allele frequency;（5） the study was published in Chinese or English;（6） the polymorphisms were detected by PCR-SSP and gene sequencing or SNaPshot SNP technology.Exclusions criteria were as follow：（1） the repeated report;（2） the data were not clear;（3） the study was based on the family;（4） the gene distribution of the control group was not inspected by the law of H-W genetic equilibrium.Results According the established inclusion criteria,6 studies with a total of 695 cases and 879 controls were identified in the SNP-607 C/A of the promoter of IL-18 gene.The SNP-137 G/C was identified in 5 studies which contained 594 cases and 781 controls.The pooled odds ratios（ORs） for studies with-137 C and-607 A loci allele variants in the IL-18 promoter gene were 1.41（95% CI： 1.15~1.74,P=0.001）and 0.93（95% CI为0.81~1.08,P=0.36）,respectively,compared with-137 G and-607 C alleles.Conclusion SNP-137G/C located in IL-18 promoter was associated with tuberculosis,and may be one of the susceptibility loci of tuberculosis.The association of SNP-607 C/A and tuberculosis susceptibility showed no statistically sig

关 键 词：结核 白细胞介素18 多态性 单核苷酸 META分析 

分 类 号：R52[医药卫生—内科学]