RT-PCR,pp65抗原及组织病理学测定肾移植术后巨细胞病毒感染的比较研究  

Comparative study of RT-PCR,pp-65 Test and histopathology for detection of CMV infection after kidney transplantation

作　　者：韩永[1] 黄海燕[1] 许晓光[1] 肖漓[1] 周文强[1] 冯凯[1] 蔡明[1] 石炳毅[1] 

机构地区：[1]解放军第309医院全军器官移植中心研究室,北京100091

出　　处：《临床军医杂志》2011年第6期1058-1061,F0003,共5页Clinical Journal of Medical Officers

基　　金：国家科技支撑计划基金项目(编号:2008BAI60B04)

摘　　要：目的比较巨细胞病毒(CMV)定量PCR检测,CMV-pp65抗原检测及组织病理学在肾移植术后CMV感染中的诊断价值。方法回顾性分析28例肾移植患者临床资料,并结合文献进行讨论。结果共有11例患者发生无临床症状的巨细胞病毒感染,经静脉注射更昔洛韦,11例全部治愈。结论 CMV定量PCR,CMV-pp65抗原检测及组织病理学均能作为肾移植术后CMV感染早期诊断的有效手段,三者比较,CMV定量PCR更敏感,CMV-pp65抗原检测更特异,组织病理学则具有创伤性,因此三者共同应用对CMV感染早期诊断更有意义。Objective To compare the efficiency of cytomegalovirus （CMV） quantitative PCR, CMV pp65 antigen test and histopathology for detection of CMV infection and their clinical significance in the recipients of renal transplantation. Methods The clinical data of 28 patients who received kidney splantation were retrospectively analyzed along with literature review. Results CMV infection occurred asymptomatically in total i 1 patients, all of them were cured through intravenous ganciclovir. Conclusion It is concluded that CMV quantitative PCR, CMV-pp65 antigen test and histopathology can detect the infection of CMV early and effectively in the recipients of renal transplantation. CMV quantitative PCR is more sensitive, and CMV-pp65 is more specific. His- topathology as golden standard is an invasive examination. The detection of CMV quantitative PCR, CMV-pp65 and histopathology are the same necessary for early diagnosis and guiding treatment of CMV infection more meaningful.

关 键 词：肾移植 巨细胞病毒感染 诊断 治疗 

分 类 号：R446.8[医药卫生—诊断学]