防己黄芪汤加减干预对阿霉素肾病大鼠肾组织MCP-1表达的影响  

Experimental Study on Effects of Addition and Subtraction of Fangjihuangqi Decoction on the Expressions of MCP-1 mRNA and Protein in Renal Cortext of Adriamycin-induced Nephropathic Rats

作　　者：陈洪宇[1] 揭乐琴[1] 王永钧[1] 汤绚丽[1] 朱晓玲[1] 

机构地区：[1]浙江省杭州市中医院(浙江中医药大学附属广兴医院)肾内科,杭州310007

出　　处：《中国中西医结合肾病杂志》2011年第11期958-962,I0001,共6页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：浙江省中医药科技项目(No.2009ZDJB02);杭州市医药卫生科技项目(No.2007B0062)

摘　　要：目的:观察探讨单核细胞趋化蛋白-1(MCP-1)在阿霉素肾病(AIN)大鼠的作用机制及防己黄芪汤加减对其表达的影响。方法:SD雄性大鼠随机分为4组,分别为正常组、模型组、防己黄芪汤+雷公藤组(A组)及防己黄芪汤-甘草+雷公藤组(B组)。造模予阿霉素(ADR)6.5mg/kg一次性尾静脉注射,正常对照予等量生理盐水尾静脉注射,注射后2周24h尿蛋白>100mg为肾病模型成立。治疗A、B组分别予防己黄芪汤+雷公藤及防己黄芪汤-甘草+雷公藤灌胃,正常组与模型组予等量生理盐水灌胃。于第1、2、8周测24h尿蛋白定量,实验4、8周时留血,取肾,检测血生化,观察肾组织病理改变,测肾组织中MCP-1表达水平。结果:1)素造模后2周,各造模组24h尿蛋白定量均高于正常对照组(P<0.05),说明阿霉素单次尾静脉注射6.5mg/kg可成功制备MCNS模型;2)免疫组化和RT-PCR检测MCP-1表达情况,模型组与正常对照组比较MCP-1明显上调,两个中药治疗组与模型对照组比较MCP-1表达下调,A、B组之间比较MCP-1表达差异无统计学意义。结论:1)阿霉素肾病大鼠存在MCP-1mRNA转录和蛋白水平的异常,MCP-1表达的增加可能是阿霉素肾病蛋白尿产生的重要因素之一;2)本研究中去除组方中的甘草对防己黄芪汤加减疗效无明显影响;3)临床上应用防己黄芪汤加减治疗慢性肾病可能是通过降低蛋白尿、改善肾病理、抑制MCP-1的表达而发挥肾保护作用。Objective：To investigate mechanism of action of MCP-1 in minimal lesion nephrotic syndrome and effects of addition an subtraction of Fangjihuangqi decoction on the expressions of MCP-1 mRNA and protein in renal cortext of adriamycin-induced nephropathic rats.Methods：The rats were randomly divided into four groups（normal control group, nephropathy control group, Fangjihuangqi decoction adding Leigongteng group, Fangjihuangqi decoction adding Leigongteng and substracting Gancao group）. ADR（6.5 mg/kg） was intravenously administered to the nephropathy group, while saline was intravenously administered to the normal control group. Two weeks after the administration, the nephropathic model of 24-hour protein urine quantity 100 mg was formed. Treatment group were given intragastric administration with Fangjihuangqi decoction adding Leigongteng and Fangjihuangqi decoction adding Leigongteng and substracting Gancao respectively, while normal control group and nephropathy control group were given intragastric administration with equivalent physiological saline. The quantitation of 24-hour proteinuria was tested on first, second and eighth week respectively. Blood and kidneys were harvested at the end of experimention. Histology of kidney was tested by HE staining. The expression levels of MCP-1 were tested by immunohistology and RT-PCR.Results：1） Two weeks after adriamycin-induced administration the quantitation of 24-hour proteinuria in the nephropathy control group increased significantly in comparison with those in the normal control group（P0.05）. This illuminated that MCNS model can be successfully prepared by Adriamycin single intravenous injection of 6.5 mg/kg. 2） The expression levels of MCP-1 were tested by immunohistology and RT-PCR. The expression levels of MCP-1 in the nephropathy control group increased in comparison with those in the normal control group. In comparison with the nephropathy control group, the expressions of MCP-1 decreased significantly in two traditional Chinese medicine-

关 键 词：防己黄芪汤加减 阿霉素肾病 单核细胞趋化因子-1 实验研究 

分 类 号：R285.5[医药卫生—中药学]