静脉铁剂影响肾性贫血小鼠组织中T淋巴细胞亚群分布的实验研究  

Effect of Intravenous Iron Injection on Distributions of T Lymphocytes Subsets,Experimental Research in Renal Anemia Mice

作　　者：谌卫[1] 荣光华[2] 赖学莉[1] 张懿[3] 郭志勇[1] 

机构地区：[1]第二军医大学附属长海医院肾内科,上海200433 [2]第二军医大学附属长征医院实验诊断科,上海200003 [3]第二军医大学附属长征医院肾内科,上海200003

出　　处：《中国中西医结合肾病杂志》2011年第11期963-966,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：2008年第二军医大学创新能力培养计划项目(No.2008066)

摘　　要：目的:观察并比较低分子右旋糖酐铁、蔗糖铁两种常用的静脉铁剂对肾性贫血小鼠不同组织中T淋巴细胞亚群分布的影响。方法:构建肾性贫血小鼠模型,尾静脉注射静脉铁剂24h后,分离小鼠外周血、肝脏、脾脏、肾脏中单个核细胞,利用流式细胞仪分析辅助性T(Thelp、T)细胞、抑制性T(Ts、Ts)细胞、自然杀伤(natural killer,NK)细胞在不同组织中的分布。结果:接受静脉铁剂后,小鼠肝脏和肾脏中Th细胞的比例下调,差异有统计学意义(P<0.05),同时铁剂在脾脏、肝脏和肾脏中还不同程度地提高T细胞向Ts细胞分化的比例,同样差异有统计学意义(P<0.05)。另外,静脉铁剂还抑制了外周血、肝脏和脾脏中NK细胞的分布,差异有统计学意义(P<0.05)。结论:静脉铁剂可以单独也可协同尿毒症一起导致机体多脏器中免疫微环境的紊乱。Objective：To compare the effects of two commercial intravenous iron agents （Low molecular weight iron dextran and iron sucrose） on distributions of T lymphocytes subsets from experimental renal anemia mice.Methods：Twenty-four hours later than iron administration via tail vein,the mononuclear cells were separated from peripheral vein blood,liver,spleen and kidney in experimental renal anemia mice.The ratios of helper T lymphocytes （Th）, suppress T lymphocytes （Ts） and natural killer cells （NK） in difference tissues were assayed by flow cytometry.Results：After the iron injection, the ratios of Th cells in liver and kidney were decreased significantly （P0.05）,meanwhile the distributions of Ts in spleen, liver and kidney were also elevated （P0.05）.And then the NK populations in peripheral blood, liver and spleen were much less than that before iron supplement（P0.05）.Conclusion：Combination with uremic status or alone, the intravenous iron agent could be result of multiple organs immune dysfunction.

关 键 词：静脉铁剂 T淋巴细胞 免疫紊乱 

分 类 号：R692.5[医药卫生—泌尿科学]