鳖甲煎丸对肺纤维化大鼠TGF-β_1及smad信号通路的影响  

Impact of Biejia Pill on Expression of TGF-β_1 and smad in Rats with Pulmonary Fibrosis

作　　者：唐志宇[1] 李天朗 唐小宾[1] 杨红[1] 

机构地区：[1]四川省德昌县人民医院

出　　处：《世界中医药》2011年第6期522-523,共2页World Chinese Medicine

摘　　要：目的:观察鳖甲煎丸抗博莱霉素所诱导的大鼠肺纤维化的作用机制。方法:SD大鼠50只,随机分为生理盐水组,模型组,醋酸泼尼松组,鳖甲煎丸中剂量组,鳖甲煎丸高剂量组。采用博莱霉素气管内注入法,制备肺纤维化模型,于实验第28天留取标本,用免疫组化技术检测TGF-β1及smad3、smad7在肺组织中的表达。结果:鳖甲煎丸中、高剂量组大鼠TGF-β1表达减弱、smad7表达增强,smad3在鳖甲煎丸高剂量组表达减弱。结论:鳖甲煎丸能够减轻博莱霉素导致的大鼠肺纤维化程度,其作用机制与调控TGF-β1的smad蛋白表达有关。Objective：To investigate mechanism of action of Biejia Pill on rats with Bleomycininduced pulmonary fibrosis. Methods： Fifty SD rats were randomized to 5 groups： model group, saline group, Prednisone Acetate group, medium dose Biejia Pill group, and high dose Biejia Pill group. The rat model of pulmonary fibrosis was established using Bleomycin. On Day28, TGF - β1, smad3 and smad7 in lung tissues were assessed using SABC methods. Results：In medium dose and high dose Biejia Pill groups, TGF - β1 expression decreased, smad7 expression increased. In high dose Biejia Pill group, smad3 expression decreased. Conclusion：Biejia Pill can reduce Bleomycin induced pulmonary fibrosis in rats, the mechanism of which is related to regulation of TGF - β1, smad3/7 expression.

关 键 词：鳖甲煎丸 肺纤维化 TGF-Β1 SMAD 

分 类 号：R619.6[医药卫生—外科学]