高效液相色谱法测定血浆中文拉法辛浓度及其在治疗药物监测中的应用  

HPLC determination of venlafaxine in human serum and TDM application

作　　者：闫小华[1] 周凡[1] 张毕奎[2] 邓小敏[1] 张宏久[1] 张璇[1] 

机构地区：[1]深圳市康宁医院,广东深圳518020 [2]中南大学湘雅三医院药剂科,长沙410013

出　　处：《中南药学》2011年第10期745-747,共3页Central South Pharmacy

摘　　要：目的建立人血中文拉法辛的测定方法,为临床应用和药动学研究提供分析手段。方法采用HPLC紫外检测法,流动相甲醇-水-四甲基乙二胺-冰醋酸(63∶37∶0.44∶0.37,v/v),Agilent Eclipse XDB-C18分离柱,UV检测波长为230nm,以安眠酮为内标,正戊烷提取。结果线性范围20～1 200μg.L-1,平均回收率为99.1%,日内日间RSD在1.1%～4.5%,且常用的其他抗抑郁药及安定类药物不干扰测定。结论本方法简单,分析速度快,灵敏度高,适用于临床血药浓度测定及药物动力学研究。Objective To develop an HPLC method for the determination of venlafaxine in human plasma,and analyse the relation between serum clozapine level and its efficacy.Methods A mobile phase was methyl alcohol-water-tetramethylethylenediamine-acetic acid（63∶37∶0.44∶0.37,v/v）,an Eclipse DXB-C18 column（150 mm×4.6 mm,5 μm） and UV detector with sensitivity of 0.01 AUFS,with methaqualone as a internal standard.The venlafaxine in the serum was extracted with pentane.The detection wavelength was 230 nm.Results The linear range was from 20 to 1 200 μg·L-1（r=0.999）,and the detection limit was 10 ng（S/N=5）.The average recovery in the serum was 99.1% and RSD was 1.1% and 4.5% for intra-day and inter-day.The optimal therapeutic concentration was estimated from 130 to 450 ng·mL-1 Conclusion This method is simple,rapid,sensitive,and reproducible.It can be used for clinical drug concentration analysis in the serum.

关 键 词：文拉法辛 血药浓度 高效液相色谱法 治疗药物监测 

分 类 号：R969.1[医药卫生—药理学] R917[医药卫生—药学]