二氢杨梅素对兔复发性口腔溃疡模型的作用  

Effect of dihydromyricetin on recurrent oral ulcer model in rabbits

作　　者：王小青[1] 陈立峰[1] 董倩倩[1] 张琼[1] 

机构地区：[1]湖南省中医药研究院中药研究所,长沙410013

出　　处：《中南药学》2011年第11期820-824,共5页Central South Pharmacy

摘　　要：目的研究二氢杨梅素(DMY)对兔复发性口腔溃疡(ROU)的作用。方法 采用同种异体口腔黏膜匀浆与弗氏完全佐剂制成的免疫抗原,给兔脊柱两侧多点皮内免疫注射,2周1次,注射5次,制备ROU模型。第5次免疫注射后7d,口腔黏膜表面给DMY 4d;末次给药后次日,取口腔黏膜做组织病理学检查,取血清测MDA含量和SOD活性,取血浆测TXB2、6K-PGFlα含量,分离淋巴细胞测CD4+和CD8+细胞数。结果兔ROU模型口腔黏膜发生炎症、坏死、溃疡等病理改变,血清SOD活性、血浆6K-PGFlα含量和CD4+数量降低,血清MDA、血浆TXB2含量和CD8+数量升高。DMY(75mg·kg-1)能使兔ROU模型口腔黏膜病理改变明显减轻;升高血清SOD活性,减少MDA生成;降低血浆TXB2含量(T)、升高6K-PGFlα含量(P),使T/P比值降低;升高CD4+细胞数,降低CD8+细胞数,使CD4+/CD8+比值增加。结论 DMY可能通过调节T淋巴细胞亚群活性、血管舒缩物质水平和抗氧化损伤而对兔复发性口腔溃疡产生作用。Objective To study the effect of dihydromyricetin （DMY） on recurrent oral ulcer （ROU） in rabbits. Methods The ROU model was established by injection of immunogen composed of the homogenate supernate of homogeneousoral oral mucosa from New Zealand rabbits and Freund＇s complete adjuvant （FCA） into rabbit backs at multi points of 2 sides of backbones subcutaneously once every 2 weeks for 5 times. Seven days after the 5th immunogen injection, DMY was given to the surface of oral mucosa for 4 d. The clay after the last administration, oral mucosa was collected for pathology. The blood serum and plasma were collected for MDA contents and SOD activity detection, TXB2 and 6K-PGF1α detection, respectively. Lymphocyte was separated for CD4＋ and CD8＋ masccline cell population detection. Results Rabbit ROU models had the pathological change of inflammation, necrosis, ulcer, etc. in the mucosa. SOD activity in the serum, 6K-PGF1α and CD4＋ in the plasma decreased while MDA in the serum, TXt32 and CD8＋ in the plasma rose. The pathological changes of the mucosa ulcer lessened obviously after the DMY administration. SOD activity increased while the production of MDA and TXB2 （T） decreased. The content of 6K-PGF1α in creased and led to T/P ratio. Mascline cell population of CD4＋ increased and CD8＋ decreased, resulting in increased ratio of CD4＋/CD8＋. Conclusion The effect of DMY on ROU in rabbits can be through regulating the activity of T lymphocyte subpopulation, the level of vasomotion materials and its antioxygen damage role.

关 键 词：二氢杨梅素 复发性口腔溃疡 超氧化物歧化酶 丙二醛 血栓烷素B2 6-酮-前列腺素Flα CD4+淋巴细胞 CD8+淋巴细胞 

分 类 号：R961.1[医药卫生—药理学]