Identification of mariner-like elements belonging to the cecropia subfamily in two closely related Helicoverpa species  

作　　者：Jianjun Wang Thomas A. Miller Yoonseong Park 

机构地区：[1]College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu Province, China [2]Department of Entomology,Kansas State University, Manhattan, Kansas [3]Department of Entomology, University of California, Riverside, California, USA

出　　处：《Insect Science》2011年第6期619-628,共10页昆虫科学（英文版）

基　　金：Acknowledgments We thank Dr. G.S. Deol （Punjab Agricultural University, India） for providing H. armigera samples from India and Dr. Fred Gould （North Carolina State University） for providing H. zea samples. We thank Dr. Kun Yan Zhu for reviewing an earlier version of this manuscript. This paper is contribution No. 10-359-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas. This work was supported by the National Natural Science Foundation of China under grant no. 30871642 and by USDA-APHIS Cooperative agreement award no. 03-8100-0879-CA.

摘　　要：Mariner transposons are widespread in eukaryote genomes and have been used as transposon vectors in insect transgenesis. We examined two closely related Helicoverpa species, the cotton bollworm Helicoverpa armigera and corn earworm Helicoverpa zea, for the presence of mariner-like elements （MLEs）. Multiple copies of two distinct MLEs, Hamarl and Hamar2, were isolated in 14. armigera, and a MLE showing a high degree of conservation to Hamarl was detected in 1-1. zea and was named Hzmarl. These MLEs belong to the cecropia subfamily, containing indels in the transposase coding region. Sequence analysis indicated the earlier invasion of Hamarl and relatively recent activity of Hamar2.Mariner transposons are widespread in eukaryote genomes and have been used as transposon vectors in insect transgenesis. We examined two closely related Helicoverpa species, the cotton bollworm Helicoverpa armigera and corn earworm Helicoverpa zea, for the presence of mariner-like elements （MLEs）. Multiple copies of two distinct MLEs, Hamarl and Hamar2, were isolated in 14. armigera, and a MLE showing a high degree of conservation to Hamarl was detected in 1-1. zea and was named Hzmarl. These MLEs belong to the cecropia subfamily, containing indels in the transposase coding region. Sequence analysis indicated the earlier invasion of Hamarl and relatively recent activity of Hamar2.

关 键 词：Helicoverpa armigera Helicoverpa zea mariner-like elements transposable elements 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治] U69[农业科学—植物保护]