Cloning and expression of the nuclear transcription factor SIβFTZ-F1 cDNA from Spodoptera fitura  

作　　者：Lin Tang Chun Zhang Lin Liu Qili Feng Sichun Zheng 

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou [2]College of Life Sciences, Hunan University of Arts and Sciences, Changde, Hunan Province, China

出　　处：《Insect Science》2011年第6期635-644,共10页昆虫科学（英文版）

基　　金：Acknowledgments This study was supported by grants of the ＂973＂ National Basic Research Program of China （2010CB 126100）, Natural Science Foundation of Guangdong Province （No. 06105204）.

摘　　要：Insect molting and metamorphosis are regulated by 20-hydroxyecdysone, which activates the ecdysone receptors, followed by expression of a series of transcription factors. Beta Fushi Tarazu-Factor 1 （βFTZ-F1） is one of these ecdysis-related earlyexpressed transcription factors. A βFTZ-F1 complementary DNA （cDNA） was cloned from Spodoptera litura. The full-length cDNA was 2 194 bp in length and the open reading frame ofS1βFTZ-F1 cDNA consisted of 1 659 bp and encoded a 552-amino acid protein with a molecular mass of 61.4 kDa. Homology analysis revealed that S1βFTZ-F 1 protein shared high identities to its homologues from other species. The A/B domains and the D domains were diverse among the insectβFTZ-F 1 proteins, but DNA binding domains （C domain） and the ligand binding domains （E domain） were highly conserved. Northern blot analysis detected a transcript of 2.2 kb in the epidermis of fifth to sixth molting larvae. Reverse transcription polymerase chain reaction revealed that SIβFTZ-F1 gene started to increasingly and periodically express before each molt, followed by a decrease during the intermolt stages.Insect molting and metamorphosis are regulated by 20-hydroxyecdysone, which activates the ecdysone receptors, followed by expression of a series of transcription factors. Beta Fushi Tarazu-Factor 1 （βFTZ-F1） is one of these ecdysis-related earlyexpressed transcription factors. A βFTZ-F1 complementary DNA （cDNA） was cloned from Spodoptera litura. The full-length cDNA was 2 194 bp in length and the open reading frame ofS1βFTZ-F1 cDNA consisted of 1 659 bp and encoded a 552-amino acid protein with a molecular mass of 61.4 kDa. Homology analysis revealed that S1βFTZ-F 1 protein shared high identities to its homologues from other species. The A/B domains and the D domains were diverse among the insectβFTZ-F 1 proteins, but DNA binding domains （C domain） and the ligand binding domains （E domain） were highly conserved. Northern blot analysis detected a transcript of 2.2 kb in the epidermis of fifth to sixth molting larvae. Reverse transcription polymerase chain reaction revealed that SIβFTZ-F1 gene started to increasingly and periodically express before each molt, followed by a decrease during the intermolt stages.

关 键 词：ECDYSIS METAMORPHOSIS MOLT Spodoptera litura transcription factor 

分 类 号：Q785[生物学—分子生物学] S852.2[农业科学—基础兽医学]