csd alleles in the red dwarf honey bee （Apis florea, Hymenoptera： Apidae） show exceptionally high nucleotide diversity  

作　　者：Zhi-Yong Liu Zi-Long Wang Xiao-Bo Wu Wei-Yu Yan Zhi-Jiang Zeng 

机构地区：[1]Honeybee Research Institute, Jiangxi Agricultural University [2]Experimental Animal Center, Institute of Occupational Disease Prevention, Nanchang, China

出　　处：《Insect Science》2011年第6期645-651,共7页昆虫科学（英文版）

摘　　要：The single locus complementary sex determination （sl-csd） gene is the primary gene determining the gender of honey bees （Apis spp.）. While the csd gene has been well studied in the Western honey bee （Apis mellifera）, and comparable data exist in both the Eastern honey bee （Apis cerana） and the giant honey bee （Apis dorsata）, no studies have been conducted in the red dwarf honey bee, Apisflorea. In this study we cloned the genomic region 3 of the A. florea csd gene from 60 workers, and identified 12 csd alleles. Analysis showed that similar to A. mellifera, region 3 of the csd gene contains a RS domain at the N terminal, a proline-rich domain at the C terminal, and a hypervariable region in the middle. However, the A. florea csd gene possessed a much higher level of nucleotide diversity, compared to A. mellifera, A. cerana and Apis dorsata. We also show that similar to the other three Apis species, in A. florea, nonsynonymous mutations in the csd gene are selectively favored in young alleles.The single locus complementary sex determination （sl-csd） gene is the primary gene determining the gender of honey bees （Apis spp.）. While the csd gene has been well studied in the Western honey bee （Apis mellifera）, and comparable data exist in both the Eastern honey bee （Apis cerana） and the giant honey bee （Apis dorsata）, no studies have been conducted in the red dwarf honey bee, Apisflorea. In this study we cloned the genomic region 3 of the A. florea csd gene from 60 workers, and identified 12 csd alleles. Analysis showed that similar to A. mellifera, region 3 of the csd gene contains a RS domain at the N terminal, a proline-rich domain at the C terminal, and a hypervariable region in the middle. However, the A. florea csd gene possessed a much higher level of nucleotide diversity, compared to A. mellifera, A. cerana and Apis dorsata. We also show that similar to the other three Apis species, in A. florea, nonsynonymous mutations in the csd gene are selectively favored in young alleles.

关 键 词：balancing selection complementary sex determination csd allele nucleotidediversity POLYMORPHISM positive selection 

分 类 号：Q78[生物学—分子生物学] S891[农业科学—特种经济动物饲养]