北美型猪蓝耳病病毒鉴别RT-PCR检测方法的建立及应用  

Establishment and Application of Identifiable RT-PCR Method for North American Type PRRSV Strains

作　　者：张燕霞[1] 吴发兴[1] 郑辉[1] 刘爽[1] 张志[1] 李晓成[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国动物检疫》2011年第12期36-38,共3页China Animal Health Inspection

基　　金：农业部财政项目--动物疫情监测与防治;广西科技攻关项目(桂科攻0815009-3-9)

摘　　要：参考Genbank发表的美洲型猪蓝耳病病毒(porcine reproductive and respiratory syndrone virus,PRRSV)CH-1a(北美型经典PRRSV)、JXA1(北美型HP-PRRSV)等毒株的的主要结构蛋白基因序列,应用Primer5.0软件设计了一对特异性的北美型PRRSV鉴别检测引物,其对经典PRRSV的扩增片段为511bp,对HP-PRRSV的扩增目片段为421bp。进行了该方法的特异性、敏感性、重复性试验,建立了美洲型PRRSV鉴别RT-PCR检测方法。应用此法对2010年1—12月收集的126份临床样品进行了检测,总体检出率达53.17﹪。结果表明该方法具有特异、敏感、重复性好等优点,可用于北美型PRRSV的临床发病鉴别检测及流行病学监测等工作。According to the Nsp2 genomic sequence of CH-la, JXA1 and the other American genotype porcine reproductive and respiratory syndrome virus （PRRSV） published in GenBank, a pair of specific and identifiable primer were designed using the Primer5.0 software, the motive segment of amplification is 511bp for classical PRRSV and 421bp for HP-PRRSV. An identifiable RTPCR method was established and its specificity , sensibility and reproducibility were tested.The method was used to detect 126 clinic samples of PRRS collected from January to December 2010, the positive rate was 53.17%, including 2 classical PRRSV and 65 HP- PRRSV. The results showed that this method was specific, sensitive, repeatable and could be used to detect and epidemiological surveillance ofAmerican-genotvoe PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 北美型 NSP2基因 RTPCR 鉴别 

分 类 号：S852.659.6[农业科学—基础兽医学]