绵羊细粒棘球蚴全血金标渗滤检测方法的建立  

Development of Dot Immunogold Filtration Assay(DIGFA)for Rapid Detection of Sheep Echinococcus granulosus Antidody with Whole Blood Sample

作　　者：余华[1] 聂华明[2] 严玉宝[1] 杨光友[2] 陈世界[1] 胡娟[1] 崔鹏博[1] 

机构地区：[1]四川出入境检验检疫局,四川成都610041 [2]四川农业大学动物医学院,四川雅安625014

出　　处：《中国动物检疫》2011年第12期39-42,共4页China Animal Health Inspection

基　　金：国家质检总局科技计划项目"动物包虫病检验检疫方法研究与规范("2009IK019)

摘　　要：为建立一种快速、高效的诊断家畜细粒棘球蚴病方法，提取绵羊细粒棘球蚴包囊新鲜囊液，盐析囊液抗原，点样于硝酸纤维膜，以胶体金-驴抗羊IgG和胶体金-兔抗鼠IgG为检测标记物，采用垂直流渗滤装置检测绵羊与人工感染细粒棘球蚴小鼠血清和全血特异性抗体。患病绵羊阳性血清及全血检出率在90．91％～94．4％，细粒棘球蚴感染小鼠血清及全血检出率均为100％；细粒棘球蚴阴性羊血清和全血假阳性率为4．00％-4．59％；与脑多头蚴病血清交叉反应率为28．57％（2／7）。研究结果表明细粒棘球蚴全血金标渗滤法（DIGFA）可应用于绵羊棘球蚴病的诊断与检疫。In order to establish a rapid and high-performance immunoassay assay in whole blood of livestock for diagnosis of hydatidosis, the cyst fluid antigen was extracted from fresh sheep Echinococcus cysts and salted-out by ammonium sulphate.The soluble cyst fluid used as antigen was dotted on nitro-cellulose and colliodal gold-donkey anti-goat IgG and colliodal gold-rabbit anti-mice IgG were employed as the probe, and a filtration device with vertical flow was applied to detect the specific IgG antibodies of sheep and mice infected with protoscoleces of Echinococcus granulosus. By using the new immunoassay, it was found that 90.91%-94.4% of whole blood and serum from sheep and 100% of whole blood and serum from mice were positive for hydatidosis, and 5% of health individuals had false positive results.However, partially high percentages of cross reactions could be demonstrated in serum of sheep with Taenia multiceps.The results showed that the dot immune gold filtration assay （DIGFA） could be used for diagnosis and quarantine inspection of livestock hydatidosis.

关 键 词：细粒棘球蚴 囊液抗原 金标免疫渗滤法 免疫诊断 绵羊 

分 类 号：S855.91[农业科学—临床兽医学]