胰胆管合流异常肝损伤抗氧化治疗的实验研究  

The experiment study of the anti-oxidative treatment in liver with anomalous pancreaticobiliary ductal union

作　　者：陈风[1,2] 吕毅[2] 汪健[3] 黄顺根[3] 王兴东[3] 

机构地区：[1]复旦大学附属华山医院南汇分院普外科,上海201300 [2]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061 [3]苏州大学附属儿童医院普外科,江苏苏州215003

出　　处：《肝胆胰外科杂志》2011年第6期481-483,486,共4页Journal of Hepatopancreatobiliary Surgery

基　　金：江苏省135工程重点人才项目(2003098)

摘　　要：目的研究胰胆管合流异常(APBDU)肝损伤的抗氧化治疗。方法采用13只健康杂种猫,建立胰胆管合流异常的动物模型后随机分为:A组7只,不进行抗氧化治疗;B组6只,抗氧化治疗;术前的13只作为对照组。饲养6个月观察肝脏的病理和肝脏过氧化产物丙二醛(maleic dialdehyde,MDA)变化。结果 13只猫均成活6个月。术前对照组13只猫的肝脏呈淡红色,质软,表面无结节;A组7只猫的肝脏均颜色加深、呈暗红色,有粟粒结节样改变,质地变韧;B组中有2只与A组猫无差别,另4只肝脏有针尖样小结节,颜色与A组比明显好转。光镜下术前对照组肝细胞结构形态正常,无细胞变性坏死,少量炎性细胞浸润;A组7只猫可见大量肝细胞气球样变,炎性细胞浸润;B组1只镜下与A组无差异,另5只气球样变细胞数目明显减少,没有坏死。电镜下B组脂滴数目、线粒体肿胀、内质网扩张程度都比A组减轻。A组MDA(4.697±1.957)nmol/mgprot,明显高于对照组(1.273±0.889)nmol/mgprot,而稍高于B组(0.789±0.860)nmol/mgprot,P=0.001,F=17.43。结论抗氧化治疗对APBDU有效。Objective To evaluate the anti-oxidative treatment in cats with anomalous pancreaticobiliary ductal union.Methods Thirteen healthy cats as control group which were made a model resembling the common channel of anomalous pancreaticobiliary ductal union in humans were divided randomly into two groups： 7 cats in group A were not treated,6 cats in group B were treated with melatonin,vitamin C and vitamin E.Six months later,the livers of these cats were removed and histopathological changes were evaluated by pathological and electron microscopic examination.The levels of MDA in livers were test.Results Thirteen cats in control group showed that the color of liver was salmon pink and no nodus in liver.In A group,the color of liver became deeper and a little nodus was found in liver.Histopathology showed ballooning changes in liver cells,mitochondria swelling and lipid droplet in intracytoplasm.One cats in group B had the same change as the group A.The other 5 cats had fewer changes than that in group A both in pathological and electron microscopic examination.The levels of MDA in livers were different between the group A,B and the control group after 6 months（4.697±1.957 nmol/mgprot,1.273±0.889 nmol/mgprot,0.789±0.860 nmol/mgprot,P=0.001,F=17.43）.Conclusion The anti-oxidative treatment is effective to the anomalous pancreaticobiliary ductal union.

关 键 词：胰胆管合流异常 肝损伤 动物模型 抗氧化 褪黑素 猫 

分 类 号：R657.3[医药卫生—外科学]