动态光散射在FOF1-ATPase制备过程中的应用  

Application of dynamic light scattering in F0F1-ATPase preparation

作　　者：李娜[1] 聂荣鑫[1] 尚桂军[1] 仓怀兴[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101

出　　处：《现代仪器》2011年第6期7-10,共4页Modern Instruments

摘　　要：采用超声波破碎茵体、DEAE阴离子柱和Superdex200分子筛柱联用法得到较纯的F0F1-ATPase全酶,并得到SDS-PAGE电泳和动态光散射检测确认。动态光散射检测显示,FOF1-ATPase分子团尺寸随温度升高而增加,不同于普通胶体和单亚基蛋白,判断与FOF1-ATPase的多亚基结构有关。研究表明,动态光散射技术是检测蛋白质均一性和稳定性的有效手段。F0F1-ATPase was obtained by use of DEAE ＆ Superdex columns after breaking Thermmicrobium roseum up with ultrasonic,and confirmed with SDS-PAGE electrophoresis ＆ dynamic light scattering（DLS）.DLS examinations revealed that the size of the F0F1-ATPase molecule blocks in the Tris-HCI buffer increased with the rising of temperature which is different from the conditions of ordinary gels.This phenomena wes deduced to be caused by the muli-subunit structure of F0F1-ATPase.Our researches showed that DLS is an effective method for detecting the mono-dispersity and stability of a protein solution.

关 键 词：F0F1-ATPase 制备 动态光散射 温度 

分 类 号：TQ641[化学工程—精细化工]