HPLC法测定烟草及烟草制品中马来酰肼残留  

Determination of Maleic Hydrazide Residue in Tobacco and Tobacco Products with High Performance Liquid Chromatography

作　　者：张洪非[1,2] 边照阳[1,2] 唐纲岭[1,2] 胡清源[1,2] 

机构地区：[1]中国烟草总公司郑州烟草研究院 [2]国家烟草质量监督检验中心

出　　处：《烟草科技》2011年第12期47-51,共5页Tobacco Science & Technology

基　　金：郑州烟草研究院院长基金项目"卷烟中农药残留燃烧后的转移行为研究"(502010CA0300);贵州省烟草专卖局项目"110种农药残留物在卷烟燃吸过程中的归趋"(D2010093)

摘　　要：建立了萃取溶剂、固相萃取小柱净化、高效液相色谱(HPLC)法检测烟草及烟草制品中马来酰肼(MH)残留量的方法,并采用该方法检测了23个烟草样品中的马来酰肼残留。结果表明:①用4 mol/L盐酸加热回流萃取1 h效果较好;②用Varian Microsorb-MV 100-5 C18色谱柱,以0.1 mol/L醋酸水溶液为流动相,等梯度洗脱,流速0.7 mL/min,检测波长313 nm,20 min内MH色谱峰和杂质色谱峰可以有效分离;③该法的回收率为97.11%,相对标准偏差为7.13%。该方法适合烟草及烟草制品中马来酰肼的测定。A method for the determination of maleic hydrazide （MH）in tobacco and tobacco products was established, in which MH in tobacco sample was extracted with a solvent, and cleaned up by a solid phase extraction cartridge, then detected with high performance liquid chromatography. The residue of MH in 23 tobacco samples was determined. The results showed that： 1）Good results were obtainable when tobacco sample was extracted with 4 mol/L hydrochloric acid under heating and refluxing for 1 hour. 2）The MH peak was completely separated from impurity peaks in 20 minutes by reversed-phase HPLC with a Varian Microsorb-MV 100-5 C18 column under isocratic elution with 0.1 mol/L acetic acid as mobile phase at a flow-rate of 0.7 mL/ min. 3）The recovery of MH was 97.11% with the relative standard deviation （RSD） of 7.13%. This method is suitable for the determination of MH in tobacco and tobacco products.

关 键 词：烟草 马来酰肼 残留 高效液相色谱法 

分 类 号：S481.8[农业科学—农药学]