基于碳纳米管和离子液体复合物修饰电极的免疫传感器检测莱克多巴胺  

An Electrochemical Immunosensor for the Determination of Ractopamine Based on Multi-walled Carbon Nanotubes and Ionic Liquid Composite

作　　者：陈昌云[1] 张红琳[1] 柳闽生[1] 颜妍[2] 赵波[2] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,南京211171 [2]南京师范大学化学与材料科学学院,南京210097

出　　处：《化学学报》2011年第23期2865-2869,共5页Acta Chimica Sinica

基　　金：江苏省教育厅高新技术产业化(No.JHZD09-18);南京大学生命分析化学教育部重点实验室开放课题(No.KLACLS08004);上海市重点科技攻关项目(No.10391901800);前沿技术研究发展计划(No.1052NM06700)资助项目

摘　　要：建立了一种新的电化学免疫传感器方法,将多壁碳纳米管(MWCNTs)和室温离子液体1-丁基-3-甲基咪唑四氟硼酸盐([BMIM]BF4)复合物,和偶联了牛血清蛋白(BSA)的莱克多巴胺抗原,使用Nafion固定在电极上,利用莱克多巴胺抗体和抗原之间特定反应的竞争模式,以K3Fe(CN)6为探针,通过循环伏安法和差分脉冲伏安法监测免疫反应,对溶液中莱克多巴胺的浓度进行检测.线性范围宽(1～1500 ng/mL),检测限可低至0.3 ng/mL.同时,我们对猪饲料实际样品进行测定,回收率令人满意.We have developed a new method of electrochemical immunosensor for the determination of ractopamine（RAC）.The modified electrodes were prepared as follows.First,the multi-walled carbon nanotubes and ionic liquid composites were cast on glassy carbon electrode（GCE）,and then the ractopamine-bovine serum albumin antigens were cast on GCE.At last,we dipped a few Nafion onto the electrode.Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication steps of immunoreaction system in solutions containing K3Fe（CN）6 as redox probes.The immunosensor demonstrates excellent RAC detection features with a broad linear range of 1 to 1500 ng/mL and a detection limit of 0.3 ng/mL.The tests with spiked animal feed samples showed excellent recovery for ractopamine.

关 键 词：多壁碳纳米管 离子液体 莱克多巴胺 电化学免疫传感器 

分 类 号：S859.84[农业科学—临床兽医学] O657.1[农业科学—兽医学]