RP-HPLC测定生脉注射液中7个人参皂苷类成分的含量  

RP-HPLC determination of seven ginsenosides in Shengmai injection

作　　者：陆娟[1,2] 李绪文[1] 魏巍[1] 杨瑞杰[1] 张培旭[1] 姚华[1] 金永日[1] 

机构地区：[1]吉林大学化学学院,长春130021 [2]长春师范学院化学学院,长春130032

出　　处：《药物分析杂志》2011年第12期2302-2304,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立RP-HPLC法同时测定生脉注射液中7个人参皂苷类成分(人参皂苷Rf、Rb1、Rg2、Rc、Rb2、Rb3和Rd)的含量。方法:使用Zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相梯度洗脱(0～15 min,29∶71;15～30min,33∶67),流速1.0 mL.min-1,检测波长203 nm,柱温30℃。结果:在本色谱条件下7个人参皂苷类成分之间有良好的分离度,各成分的浓度和相应的峰面积之间呈现良好的线性关系(r=0.9999,n=7),精密度、重复性及加样回收率的RSD均小于3%。结论:本方法可同时测定生脉注射液中7个人参皂苷类成分的含量,可作为生脉注射液的质量控制手段。Objective：To establish an RP-HPLC method for simultaneous determining seven ginsenosides（ginsenoside Rf,Rb1,Rg2,Rc,Rb2,Rb3 and Rd） in Shengmai injection.Methods：Using a Zorbax SB-C18column（4.6 mm×250 mm,5 μm）,acetonitrile-water as amobile phase（0-15 min,29∶ 71;15-30 min,33∶ 67） at 30 ℃.The flow rate was 1 mL·min-1 and the detection wavelength was set at 203 nm.Results：The relationship between the concentrations and the peak areas of seven ginsenosides was linear respectively（r=0.9999,n=7）.The RSDs of precision,repeatability and recovery were all less than 3%.Conclusion：The method can be used to determine seven components simultaneously in Shengmai injection and can be applied to the quality control of Shengmai injection.

关 键 词：生脉注射液 反相高效液相色谱 人参皂苷 多组分测定 

分 类 号：R917[医药卫生—药物分析学]