谷胱甘肽在咖啡酸修饰碳糊电极上的电催化氧化及电分析方法  

Electrocatalytic oxidation of glutathione at caffeic acid modified carbon paste electrode and its electrochemical determination

作　　者：张艳梅[1] 段成茜[1] 高作宁[1] 

机构地区：[1]宁夏大学能源化工重点实验室化学化工学院,银川750021

出　　处：《药物分析杂志》2011年第12期2308-2311,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:研究了谷胱甘肽(还原型,glutathione,GSH)在咖啡酸(Caffeic acid,CFA)修饰碳糊电极(CFA/CPE)上的电催化氧化行为和电化学分析方法。方法:循环伏安法(CV),计时电流法(CA)和线性扫描伏安法(LSV)。结果:GSH在碳糊电极(CPE)上的直接电化学氧化过程十分迟缓,CFA/CPE对GSH电化学氧化具有良好的催化作用。同时测定了GSH在CFA/CPE上的电极过程动力学参数,用LSV法测得催化氧化峰电流与GSH在5.0×10-5～1.0×10-3 mol.L-1浓度范围内呈良好线性关系,线性回归方程为Ipa(μA)=2.003c(10-3 mol.L-1)+3.448,r=0.9989,检出限为4.0×10-5 mol.L-1。结论:CFA/CPE对GSH电化学氧化具有良好的催化作用,该方法可用于市售还原型谷胱甘肽药物含量的电化学定量测定。Objective：Electrocatalytic oxidation behaviors and electrochemical determination of glutathione（GSH） at carbon paste electrode（CPE） and caffeic acid modified carbon paste electrode（CFA/CPE） were investigated.Methods：Cyclic voltammetry（CV）,chronoamperometry（CA） and linear sweep voltammetry（LSV）.Results：It was found that glutathione itself showed a poor electrochemical response at carbon paste electrode（CPE）,the electrochemical behaviors could be greatly enhanced by using CFA/CPE,which enables a sensitive electrochemical determination of the substrate glutathione.The kinetic parameters of the reaction were evaluated.The catalytic oxidation peak currents of glutathione versus its concentration had a good linear relationship in the concentration range of 5.0×10-5~1.0×10-3 mol·L-1 with the correlation coefficient of 0.9989,and the detection limit of 4.0×10-5 mol·L-1by LSV.Conclusion：CFA/CPE can catalyze the oxidation of GSH well.Furthermore,the proposed method can be applied in the electrochemical determination of glutathione content with real samples with the simple manipulation.

关 键 词：谷胱甘肽 咖啡酸 碳糊修饰电极 电催化氧化 电分析方法 

分 类 号：R917[医药卫生—药物分析学]