HPLC测定枸橼酸莫沙必利片的有关物质  

HPLC determination of related substances in mosapride citrate tablets

作　　者：王守箐[1] 

机构地区：[1]临沂大学生命科学学院,临沂276003

出　　处：《药物分析杂志》2011年第12期2330-2332,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱测定枸橼酸莫沙必利片有关物质的方法。方法:采用C18柱(250 mm×4.6 mm,5μm),以0.05mol.L-1枸橼酸溶液(用氢氧化钠溶液调节pH至4.0)-乙腈(65∶35)为流动相,流速1.0 mL.min-1,检测波长为274 nm,自身对照法测定。结果:线性范围为0.502～4.016μg.mL-1,r2=0.9978,最低检测限为0.33 ng。结论:该方法准确、简便,可以用于枸橼酸莫沙必利片有关物质的测定。Objective：To establish an HPLC method for the determination of related substances in mosapride citrate tablets.Methods：The separation was performed on C18Column（250 mm×4.6 mm,5 μm） with mobile phase consisted of 0.05 mol·L-1 citric acid（adjusted pH=4.0 with sodium hydroxide solution）-acetonitrile（65∶ 35）and the flow rate was 1.0 mL·min-1.The detection wave length was set at 274 nm.The content of related substance was calculated by self-control with main component.Results：The standard curve was linear within the range of 0.502-4.016 μg·mL-1（r2=0.9978） and the limit of detection was 0.33 ng.Conclusion：The method is accurate and simple for the determination of the related substances in mosapride citrate tablets.

关 键 词：枸橼酸莫沙必利 片剂 有关物质 主成分自身对照法 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]