肺动脉高压大鼠肺小动脉内皮细胞间和右心室心肌中缝隙连接蛋白43表达变化的初步研究  

Preliminary Study of Connexin43 Expression at Small Pulmonary Artery and Right Ventricle in Rats With Pulmonary Arterial Hypertension

作　　者：谭晓燕[1] 何建国[2] 

机构地区：[1]中国医学科学院北京协和医学院心血管病研究所阜外心血管病医院心内科,北京市100037 [2]中国医学科学院北京协和医学院心血管病研究所阜外心血管病医院肺血管病诊治中心,北京市100037

出　　处：《中国循环杂志》2011年第6期469-472,共4页Chinese Circulation Journal

基　　金：高等学校博士学科点专项科研基金资助课题;课题编号:20060023042

摘　　要：目的:研究肺动脉高压大鼠及波生坦(bosentan)干预后肺小动脉内皮细胞间及右心室心肌中缝隙连接蛋白(Cx)43表达的变化,并探究其意义。方法:10只SD大鼠为正常对照组(n=10),不做任何处理;其余57只通过腹腔内注射野百合碱(60 mg/kg)两周后,随机将大鼠分成2组,肺动脉高压SD大鼠+安慰剂组(n=35),肺动脉高压SD大鼠+波生坦组(n=22),予以安慰剂或波生坦300 mg/(kg·d)治疗,每天一次,共计3周。通过免疫荧光的方法测定Cx43在肺小动脉内皮细胞间、右心室心肌组织中表达的变化。结果:在正常对照组的肺小动脉内皮细胞间未检测到Cx43,但在肺动脉高压SD大鼠+安慰剂组中肺小动脉内皮细胞间出现了Cx43的异常表达,肺动脉高压SD大鼠+波生坦组Cx43的表达水平显著下降,三组之间两两比较差异均有统计学意义(P<0.01)。在右心室心肌纵切面,正常对照组的Cx43多数集中于与心肌细胞长轴垂直的端端连接处,少数位于与长轴平行的细胞侧侧连接处;而在肺动脉高压SD大鼠+安慰剂组Cx43的表达在端端连接处明显减少,在侧侧连接处明显增加;肺动脉高压SD大鼠+波生坦组Cx43的表达与正常对照组相似。三组单位面积的右心室心肌组织Cx43的表达面积的半定量分析差异无统计学意义(P>0.05)。结论:肺小动脉内皮细胞间Cx43的异常表达在肺动脉高压的发病机制中可能起着非常重要的作用。右心室心肌Cx43的分布紊乱可能是导致右心室重塑的机制之一。Objective ：To study the effect of bosentan for connexin43 （Cx43） expression in endothelial cells of small pulmonary artery and in myocytes of right ventricle （RV） in rats with pulmonary arterial hypertension （PAH）. Methods：PAH rats＇ model was created by monocrotaline （60 mg/kg） injection for 2 weeks,then PHA rats were divided in two groups, PAH＋vehicle group （n = 35 ）, and PAH＋bosentan group（ n = 22）, in which the rats received bosentan 300 mg/（ kg · day） for 3 weeks. In addition,10 normal rats were set as Control group. Cx43 expression in Small pulmonary artery endothelial cells and in RV myocytes were examined by immuno fluorescence assay. Results：For small pulmonary artery, there was no Cx43 expression detected in endothelial cells in Control group, while it was found in both PAH＋vehicle group and in PAH＋bosentan group, and Cx43 expression was significantly lower in PAH＋bosentan group than that in PAH＋vehicle group,P〈0. 01. For RV myocytes,in Control group, most of Cx43 staining was aggregated at thecell termini, the staining was weak at the side to side cell junctions ; while in PAH＋vehicle group, Cx43 expression was decreased at the cell termini and increased at the side to side area; in PAH＋bosentan group, Cx43 expression was similar to Control group. Conclusion： In PAH rats, the abnormal expression of Cx43 in small pulmonary artery endothelial cells might be associated with PAH occurrence. Perturbation of Cx43 in RV myocytes could be one of the reasons for RV remodel.

关 键 词：肺动脉高压 缝隙连接蛋白 内皮细胞 右心室心肌 

分 类 号：R54[医药卫生—心血管疾病]