牛精子冷冻损伤研究进展  

Advances in Damage Mechanism of Cryopreservation of Bull Semen

作　　者：王红[1] 胡建宏[1] 侯丽鹏[1] 索丽娟[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国畜牧兽医》2011年第12期125-128,共4页China Animal Husbandry & Veterinary Medicine

基　　金：奶牛高活力冻精生产关键技术中试与示范(2010GB2G000466);陕西省"13115"科技创新工程计划项目(2010ZDGC-01);秦川肉牛分子细胞工程育种技术创新与优势性状新品系培育(PY200901)

摘　　要：人工授精技术是目前通过冷冻精液进行牛遗传改良而应用最为广泛的生物技术。虽然如此,由于精液产品的质量而导致人工授精的遗传影响被限制,在精液的冷冻-解冻过程中40%～50%的活精子失去完整性或功能受到损伤。作者在参阅国内外相关文献的基础上,通过阐述冷冻-解冻对精子细胞的损伤作用及机理、精浆对牛冷冻精液质量的影响、冷冻-解冻过程中精子的过氧化损伤,以及冷冻-解冻后精子相关基因与蛋白质的变化等,以期为牛高质量冷冻精液的研究提供一定参考,进一步提升冷冻精液质量。Artificial insemination（AI） is the most widely used biotechnology for genetic improvement through banks of frozen spermatozoa in bull.However,the genetic impact in the AI industry is limited by the efficiencies of semen production,40% to 50% of viable sperm lose their integrity or functionality during the freezing-thawing process.Based on the literature from home and abroad in recent years,the article explained the effects and mechanism of frozen-thawed on spermatozoa,the influence of seminal plasma on bull frozen sperm quality,the peroxide damage and the protein and gene changes during frozen-thaw.The aim was to provide a reference for research of bull high quality semen and further promote the frozen semen quality.

关 键 词：牛 精液冷冻 损伤机理 研究进展 

分 类 号：S814.4[农业科学—畜牧学]