广西沼泽型水牛血清蛋白多态性的研究  

Study on Serum Protein Polymorphisms in Guangxi Swamp Buffaloes

作　　者：邓继贤[1] 杨秀荣[2] 何荆洲[3] 蒋和生[1,2] 

机构地区：[1]广西科学院,中国-东盟(广西)生物技术合作研发中心,广西南宁530003 [2]广西大学动物科学技术学院,广西南宁530004 [3]广西农业科学院生物技术研究所,广西南宁530007

出　　处：《中国畜牧兽医》2011年第12期129-133,共5页China Animal Husbandry & Veterinary Medicine

基　　金：桂科攻(0537012-C);桂财教函((2009)234号);桂科能(0992028-967)

摘　　要：本研究采用聚丙烯酰胺凝胶电泳技术对193头广西西林水牛和196头广西富钟水牛的血清运铁蛋白、白蛋白和淀粉酶的基因型、基因型频率及遗传变异系数进行分析,探讨这两个品种的遗传背景。结果表明,西林水牛和富钟水牛运铁蛋白(Tf)受TfA和TfD两个等位基因控制,基因频率TfD>TfA,以TfD为优势基因,DD为优势基因型;西林水牛和富钟水牛的白蛋白(Alb)受AlbA和AlbX两个等位基因控制,基因频率为AlbX>AlbA,以AlbX为优势基因,西林水牛以XX为优势基因型,而富钟水牛以AX为优势基因型;富钟水牛血清淀粉酶(Amy)存在AA、AB、BB、BC、AC、CC 6种基因型,受AmyA、AmyB、AmyC 3个等位基因控制,在AmyⅠ、AmyⅡ中都是以AmyA为优势基因,分别以AB和BC为优势基因型。本研究结果发现,3种血清蛋白在西林水牛和富钟水牛中不存在性别差异(P>0.05),提示广西这两个优良地方水牛品种的遗传稳定性较好。193 Guangxi Xinlin and Fuzhong buffalo blood samples were collected in this study.Polyacrylamide gel electrophoresis was employed to exam the serum transferring（Tf）,serum albumin（Alb）,and amylase（Amy）.The genotype,frequency of genotype,and genetic variation coefficient were analyzed.The results showed that TfA and TfD alleles were found in Xilin and Fuzhong buffalo,the gene frequency of TfDTfA.TfD was dominant genes and DD was dominant genotype in two buffaloes.There were AlbA and AlbX alleles in two buffaloes and the gene frequency of AlbXAlbA.Meanwhile,Xilin buffalo had a higher XX and lower AX genotypes comparing with Fuzhong buffalo,respectively.There were six genotypes of AA,AB,BB,BC,AC,and CC which were controlled by three alleles of AmyA,AmyB,and AmyC,respectively.AmyA and AC were a dominant gene and genotype in Amy Ⅰ and Amy Ⅱ,respectively.Three proteins were not sex specific and the variants were a little.

关 键 词：西林水牛 富钟水牛 血清蛋白 多态性 

分 类 号：Q753[生物学—分子生物学]