豫西脂尾羊血液蛋白的多态性研究  

Study on Polymorphisms of Blood Proteins in Yuxi Fat-tailed Sheep

作　　者：白俊艳[1] 赵淑娟[1] 王玉琴[1] 庞有志[1] 邢静[1] 武小宝[1] 朱宇翔[1] 李宏伟[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《中国畜牧兽医》2011年第12期134-137,共4页China Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目(0624230021);河南科技大学博士科研启动基金(09001094)

摘　　要：本研究利用聚丙烯酰胺凝胶电泳技术(PAGE)分析了河南豫西脂尾羊的血红蛋白(Hb)、转铁蛋白(Tf)、红细胞蛋白质(Ep)、前白蛋白(Pa)和血清酯酶(Es)的多态性。结果表明,在豫西脂尾羊群体中Hb存在3种基因型为HbAA、HbAB、HbBB,它们受HbA和HbB两个共显性等位基因控制,其中HbAA基因型频率为0.0571,HbAB为0.6286,HbBB为0.3143;HbA等位基因频率为0.3714,HbB为0.6286。Tf存在3种基因型为TfAA、TfAB、TfBB,它们受TfA和TfB两个共显性等位基因控制,其中TfAA基因型频率为0.2000,TfAB为0.2545,TfBB为0.5455;TfA等位基因频率为0.3273,TfB为0.6727。Ep有EpAA和EpAa共2种基因型,其中EpAA基因型频率为0.4286,EpAa为0.5714;EpA基因频率为0.7143,Epa为0.2857。Es基因座有2个等位基因即Es+和Es-,而且Es+和Es-的基因频率分别为0.5500和0.4500,有3种基因型Es++、Es+-、Es--的频率分别为0.4250、0.2500和0.3250。Pa上未检测到多态性,它呈现单态。Yuxi fat-tailed sheep in Hennan were used to investigate the polymorphism of hemoglobin（Hb）,transferrins（Tf）,erythrocyte proteins（Ep）,preceding-album（Pa） and arylesterase（Es） by discontinuous polyacrylamide gel electrophoresis（PAGE）.The results showed that there were three genetypes including HbAA,HbAB,HbBB on the locus of hemoglobin which were controlled by two allelic genes HbA and HbB in Yuxi fat-tailed sheep populations.The genotypic frequency of HbAA,HbAB,HbBB were 0.0571,0.6286,0.3143.The gene frequency of HbA and HbB were 0.3714 and 0.6286.There were three gene types including TfAA,TfAB,TfBB on the locus of transferrins which were controlled by two allelic genes TfAand TfB in Yuxi fat-tailed sheep populations.The genotypic frequency of TfAA,TfAB,TfBB were 0.2000,0.2545,0.5455.The gene frequency of TfA and TfB were 0.3273 and 0.6727.The research discovered that it had examined the multi-state properties in the Ep gene place,it had the EpAA and the EpAa two genetypes.The genotypic frequency of EpAA,EpAa were 0.4286 and 0.5714.The gene frequency of EpA and Epa were 0.7143,0.2857.The results showed that the Yuxi fat-tailed sheep Es loci had two alleles that Es＋ and Es-,and the Es＋ and Es-gene frequency 0.5500 and 0.4500 for the other,there were three kinds genotypes of Es＋＋,Es＋-,Es——,the frequency were 0.4250,0.2500,and 0.3250,respectively.The research discovered that it had not examined the multi-state properties in the preceding-album gene place.

关 键 词：豫西脂尾羊 血液蛋白 血红蛋白 转铁蛋白 血清酯酶 

分 类 号：S826.9[农业科学—畜牧学]