黄白口服液的质量控制研究  

Study on the Quality Standard of Huangbai Oral Liquid

作　　者：王胜义[1] 齐志明[1] 刘治岐[2] 荔霞[1] 董书伟[1] 刘世祥[1] 刘永明[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省中兽药工程技术研究中心,甘肃兰州730050 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中国畜牧兽医》2011年第12期226-231,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划子课题项目(2006BAD04A05);中央级公益性科研院所基本科研业务费专项资金项目(BRF110301)

摘　　要：为了制定黄白口服液质量标准,采用薄层色谱(TLC)法对组方中的黄连、金银花、黄芩、地榆炭进行定性鉴别;用高效液相色谱(HPLC)法对制剂中盐酸小檗碱和黄芩苷进行含量测定。结果TLC鉴别专属性强,重现性好;盐酸小檗碱线性范围在0.139～0.834μg(R2=0.9999),平均回收率为97.04%,RSD为0.97%(n=6);黄芩苷线性范围0.235～1.41μg(R2=0.9999),平均回收率为97.64%,RSD为0.71%(n=6)。对黄连、金银花、黄芩、地榆炭的定性鉴别及盐酸小檗碱和黄芩苷的含量测定可用来进行制剂的质量控制。To establish a quality control standard for Huangbai oral liquid,Rhizoma coptidis,Flos lonicerae,Radix scutellariae and charred radix sanguisorbae were identified by TLC.Content of Berberine hydrochloride and Baicalin were determinated by HPLC.The results showed that the TLC identification was highly specific and the spots were clear and focused with good reproducibility.The calibration curve of Berberine hydrochloride was linear in the range of 0.139 to 0.834 μg（R2=0.9999）.The average recovery rate was 97.04%,RSD=0.97%（n=6）.The calibration curve of Baicalin was linear in the range of 0.235 to 1.41 μg（R2=0.9999）.The average recovery rate was 97.64%,RSD=0.71%（n=6）.The quality of the preparation could be controlled by qualitative identification of Coptis,honeysuckle,Scutellaria,Sanguisorba carbon and content determination of Berberine hydrochloride and Berberine.

关 键 词：黄白口服液 薄层鉴别 含量测定 质量标准 

分 类 号：S859.7[农业科学—临床兽医学]